PMID: 3754904Feb 20, 1986Paper

Minus-strand initiation by brome mosaic virus replicase within the 3' tRNA-like structure of native and modified RNA templates

Journal of Molecular Biology
W A MillerT C Hall

Abstract

An RNA-dependent RNA polymerase (replicase) extract from brome mosaic virus-infected barley leaves has been shown to initiate synthesis of (-) sense RNA from (+) sense virion RNA. Initiation occurred de novo, as demonstrated by the incorporation of [gamma-32P]GTP into the product. Sequencing using cordycepin triphosphate to terminate (-) strands during their synthesis by the replicase generated sequence ladders that confirmed that copying was accurate, and that initiation occurred very close to the 3' end. The precise site of initiation was further defined by testing the replicase template activity after stepwise removal of 3'-terminal nucleotides. Whereas removal of the terminal A did not decrease template activity, removal of the next nucleotide (C-2) did. Thus, initiation almost certainly occurs opposite the penultimate 3'-nucleotide (C-2) in vitro. The structure of the double-stranded replicative form of RNA isolated from brome mosaic virus-infected leaves was consistent with such a mechanism occurring in vivo, in that it lacked the 3'-terminal A found on virion RNAs. The specific site of (-) strand initiation and normal template activity were retained for RNAs with as many as 15 to 30 A residues added to the 3' end. Howeve...Continue Reading

References

Nov 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·F R Kramer, D R Mills
Oct 1, 1977·Nature·A SimoncsitsH Guilley
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J J BujarskiT C Hall
Jan 1, 1980·Methods in Enzymology·T E EnglandO C Uhlenbeck
Jul 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J HaseloffP Kaesberg
Dec 15, 1981·Journal of Molecular Biology·F MeyerC Weissmann
Oct 10, 1980·Nucleic Acids Research·M G Murray, W F Thompson
Nov 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·P AhlquistL S Loesch-Fries

❮ Previous
Next ❯

Citations

Dec 11, 2007·Archives of Virology·S L Calhoun, A L N Rao
Jan 1, 1993·Biochimie·R GiegéT W Dreher
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A M Dzianott, J J Bujarski
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A L RaoT C Hall
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·P A PowellR N Beachy
Oct 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J MéndezM Salas
Jul 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·P D Nagy, J J Bujarski
Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·L A Ball
Oct 29, 1996·Proceedings of the National Academy of Sciences of the United States of America·R Giegé
Jun 11, 1992·Nucleic Acids Research·N Rodriguez-Cousiño, R Esteban
Jun 1, 1993·The Plant Cell·S P Dinesh-Kumar, W A Miller
Oct 15, 1991·European Journal of Biochemistry·R M MansL Bosch
Mar 30, 2005·Journal of Virology·Richard W Hardy, Charles M Rice
Nov 10, 2001·Annual Review of Phytopathology·Theo W. Dreher

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.