MiR-101-containing extracellular vesicles bind to BRD4 and enhance proliferation and migration of trophoblasts in preeclampsia.

Stem Cell Research & Therapy
Jinhui CuiPing Li

Abstract

Preeclampsia (PE) is a frequently occurring pregnancy disorder in the placenta, which results in various maternal and fetal complications. The current study aims to evaluate the role of extracellular vesicles (EVs)-encapsulated microRNA (miR)-101 in biological processes of trophoblasts in PE and its underlying mechanism. Human umbilical cord mesenchymal stem cell (HUCMSC) and HUCMSC-derived EVs were isolated and cultured, after which EV characterization was carried out using PKH67 staining. In silico analyses were adopted to predict the downstream target genes of miR-101, and dual luciferase reporter gene assay was applied to validate the binding affinity. Furthermore, loss- and gain-of-function approaches were adopted to determine the role of miR-101 and bromodomain-containing protein 4 (BRD4) in trophoblast proliferation and invasion using EDU staining and transwell assay. In addition, a rat model of PE was established to verify the function of EV-encapsulated miR-101 in vivo. Placental tissues obtained from PE patients presented with downregulated miR-101 expression and upregulated BRD4 and CXCL11 expression. EV-encapsulated miR-101 from HUCMSCs could be delivered into the trophoblast HTR-8/SVneo cells, thus enhancing prolif...Continue Reading

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Citations

Oct 21, 2020·Epigenomics·Srivathchava SelvarajDurairaj Sekar
Oct 4, 2020·BJOG : an International Journal of Obstetrics and Gynaecology·Yuanyuan ChenFengxia Xue
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Mar 30, 2021·Biology of Reproduction·Zheng WangJinjun Liu

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Datasets Mentioned

BETA
GM130

Methods Mentioned

BETA
light microscopy
transmission electron microscopy
PCR
Assay
cesarean section
flow cytometry
transfection

Software Mentioned

Coexpedia
StarBase
RNAInter
KOBAS
- Matrix ( MEM )
MEM
ImageJ
TargetScan
Multi
SPSS

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