miR-106b-5p inhibits the invasion and metastasis of colorectal cancer by targeting CTSA

OncoTargets and Therapy
Shujuan NiWeiqi Sheng

Abstract

Although miR-106b-5p has been reported to play a pivotal role in various human malignancies, its role in colorectal cancer (CRC) remains unknown. In this study, we comprehensively investigated miR-106b-5p expression and biologic functions in CRC and the molecular mechanism involved. miR-106b-5p expression was detected in CRC tissues and cell lines by quantitative reverse transcription-polymerase chain reaction. The effects of miR-106b-5p on metastasis were determined in vitro using transwell assays, and in vivo effects were evaluated using a mouse tail vein injection model. Downstream targets of miR-106b-5p were confirmed using bioinformatics programs, luciferase assays, and rescue experiments. Target gene expression and clinicopathologic parameters were also analyzed. miR-106b-5p expression was lower in CRC tissues than in corresponding nontumorous tissues (P=0.009), and miR-106b-5p downregulation was negatively associated with lymph node metastasis (P=0.006). Functional assays demonstrated that miR-106b-5p overexpression suppressed CRC cell migration and invasion in vitro and lung metastasis formation in vivo. In addition, luciferase assays confirmed that miR-106b-5p directly bound to the 3' untranslated region of cathepsin A...Continue Reading

Citations

Jun 20, 2019·Cell Cycle·Surinder M SoondAndrey A Zamyatnin
Sep 26, 2020·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Seokhwi KimDakeun Lee
Sep 15, 2020·Cancer Biomarkers : Section a of Disease Markers·Tao JiXiaoming Zhang
Apr 24, 2020·International Journal of Molecular Medicine·Heming ChenHongyan Tian
Feb 26, 2020·BMC Medical Genomics·Md Altaf-Ul-AminShigehiko Kanaya
Jan 14, 2021·International Journal of Molecular Sciences·Han Yeoung LeeJong Kook Park
May 16, 2021·Journal of Hematology & Oncology·Ziqi ZhangXiawei Wei

❮ Previous
Next ❯

Methods Mentioned

BETA
surgical resection
transfection
electrophoresis

Software Mentioned

TargetScan
GraphPad Prism
SPSS
Miranda

Related Concepts

Related Feeds

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Related Papers

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Ke WeiYijiang Chen
Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Jing LiMing-Xia Yan
© 2022 Meta ULC. All rights reserved