miR-10a rejuvenates aged human mesenchymal stem cells and improves heart function after myocardial infarction through KLF4

Stem Cell Research & Therapy
Jun DongJiao Li

Abstract

Aging is one of the key factors that regulate the function of human bone marrow mesenchymal stem cells (hBM-MSCs) and related changes in microRNA (miRNA) expression. However, data reported on aging-related miRNA changes in hBM-MSCs are limited. We demonstrated previously that miR-10a is significantly decreased in aged hBM-MSCs and restoration of the miR-10a level attenuated cell senescence and increased the differentiation capacity of aged hBM-MSCs by repressing Krüpple-like factor 4 (KLF4). In the present study, miR-10a was overexpressed or KLF4 was downregulated in old hBM-MSCs by lentiviral transduction. The hypoxia-induced apoptosis, cell survival, and cell paracrine function of aged hBM-MSCs were investigated in vitro. In vivo, miR-10a-overexpressed or KLF4-downregulated old hBM-MSCs were implanted into infarcted mouse hearts after myocardial infarction (MI). The mouse cardiac function of cardiac angiogenesis was measured and cell survival of aged hBM-MSCs was investigated. Through lentivirus-mediated upregulation of miR-10a and downregulation of KLF4 in aged hBM-MSCs in vitro, we revealed that miR-10a decreased hypoxia-induced cell apoptosis and increased cell survival of aged hBM-MSCs by repressing the KLF4-BAX/BCL2 path...Continue Reading

References

Jun 7, 2008·Circulation Research·Stefanie Dimmeler, Annarosa Leri
Oct 28, 2008·Neoplasia : an International Journal for Oncology Research·Louis CheslerWilliam A Weiss
May 28, 2010·PloS One·Nicole Noren HootenMichele K Evans
Jan 20, 2011·Molecular Therapy : the Journal of the American Society of Gene Therapy·Sun Hwa KimDonghoon Choi
Mar 2, 2012·Gene Therapy·M Giacca, S Zacchigna
May 2, 2012·Journal of Hematology & Oncology·Shihua WangRobert Chunhua Zhao
Sep 18, 2012·Haematologica·Xiang LiA Mario Marcondes
Dec 1, 2012·Stem Cells Translational Medicine·Yunshan ZhaoBartley P Griffith
Jan 12, 2013·Journal of Cellular and Molecular Medicine·Claudia PennaPasquale Pagliaro
May 23, 2013·Journal of Cellular Physiology·Jiao LiShi-Ming Liu
May 31, 2013·Journal of Cardiovascular Pharmacology·Zhen-hui ZhangShi-ming Liu
Apr 8, 2014·Pharmacology & Therapeutics·Daniel I BromageDerek M Yellon
Feb 27, 2017·Gene·Amr M Ghaleb, Vincent W Yang

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Citations

Jan 23, 2020·Cellular and Molecular Life Sciences : CMLS·Xing-Liang FanQing-Ling Fu
Nov 5, 2019·Current Stem Cell Research & Therapy·L Vinod Kumar ReddyDwaipayan Sen
Jan 8, 2019·Cancer Biomarkers : Section a of Disease Markers·Dongliang HouLi Chen
Jul 12, 2019·World Journal of Stem Cells·Juan Antonio Fafián-LaboraMaría C Arufe
Jun 26, 2020·Frontiers in Cell and Developmental Biology·Xueke ZhouXin Li
Oct 10, 2020·World Journal of Stem Cells·Qing-Shu MengXiao-Ting Liang
Feb 12, 2021·Science Translational Medicine·Bohao LiuGordana Vunjak-Novakovic
Dec 16, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Ruixi LuoYounan Chen
Apr 6, 2021·Frontiers in Cell and Developmental Biology·Jingyi CaiZhihe Zhao
Apr 18, 2021·Stem Cell Reviews and Reports·Saravanan RameshMurugan Ramalingam
Aug 25, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Soudeh Ghafouri-FardAfshin Taheriazam

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Methods Mentioned

BETA
flow cytometry
PCR
protein assay
enzyme-linked immunosorbent assay
ELISA
antisense oligonucleotide
X-ray

Software Mentioned

GraphPad InStat
ImageJ

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