miR-122-5p as a plasma biomarker of liver injury in fish exposed to microcystin-LR

Journal of Fish Diseases
M FlorczykA Łakomiak

Abstract

Recent studies have shown the presence of large amounts of microRNAs (miRNAs; miRs) from damaged cells in the peripheral blood. In this study, we investigated the levels of miRNAs circulating in the blood plasma of whitefish (Coregonus lavaretus) after exposure to microcystin-LR. We used real-time PCR to examine the relative expression of plasma levels of 4 miRNAs (miR-122-5p and let-7c-5p, the liver-enriched microRNAs, miR-148a-3p which promotes the hapatospecific phenotype in mammals, and miR-92a-3p, a cell proliferation and angiogenesis promoter, potentially hepatocarcinogenic) during the first 48 h after exposure to MC-LR. We observed a rapid increase of miR-122-5p levels 8 h after exposure (P < 0.05), which continued to the end of the experiment. Our results demonstrated that the plasma miR-122-5p was indicative of MC-LR-induced liver injury, exhibiting areas under the curve close to 1 in ROC analysis (AUC = 0.976, P < 0.001). Although plasma levels of miR-148a-3p and miR-92a-3p were significantly elevated by the end of the experiment, their discriminative power was lower than reported for the miR-122-5p. Based on these results and reports on miRNA-based diagnosis of liver injuries in mammals, plasma miR-122-5p could be co...Continue Reading

References

Aug 5, 1998·Toxicon : Official Journal of the International Society on Toxinology·R M Dawson
Jul 11, 2001·Drug Safety : an International Journal of Medical Toxicology and Drug Experience·N Kaplowitz
Jul 10, 2002·Veterinary Pathology·R E Guzman, P F Solter
Aug 2, 2003·The New England Journal of Medicine·William M Lee
Oct 29, 2004·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·Kenneth BurhopTimothy Estep
Nov 9, 2004·Toxicon : Official Journal of the International Society on Toxinology·Xiao-Yu LiJin-Ae Lee
Jan 22, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Rahul A NathwaniNeil Kaplowitz
Aug 29, 2007·Clinics in Liver Disease·John R Senior
Jan 15, 2008·Toxicon : Official Journal of the International Society on Toxinology·Darío AndrinoloMaría J T de Alaniz
Feb 23, 2008·Toxicology·Josef OzerShelli Schomaker
Mar 14, 2008·Journal of Medical Toxicology : Official Journal of the American College of Medical Toxicology·Maryann Mazer, Jeanmarie Perrone
Sep 6, 2008·PloS One·Shlomit GiladAyelet Chajut
Feb 28, 2009·Proceedings of the National Academy of Sciences of the United States of America·Kai WangDavid J Galas
Apr 21, 2009·Toxicon : Official Journal of the International Society on Toxinology·Paweł BrzuzanStefan Dobosz
Sep 12, 2009·Clinical Chemistry·Omar F LaterzaWarren E Glaab
Oct 14, 2009·Toxicological Sciences : an Official Journal of the Society of Toxicology·Minghua WangDazhi Wang
Jun 4, 2010·Pathology International·Masatoshi ShigokaMasahiko Kuroda
Feb 19, 2011·Mutation Research·Enikö Sonkoly, Andor Pivarcsi
Jul 1, 2011·Toxicological Sciences : an Official Journal of the Society of Toxicology·Tsuyoshi Yokoi, Miki Nakajima
Sep 21, 2011·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Waqar R R FaridLuc J W van der Laan
Nov 13, 2012·Toxicologic Pathology·Sofie StarckxLieve Lammens
Mar 20, 2013·Toxicon : Official Journal of the International Society on Toxinology·Daniela SedanDarío Andrinolo
Apr 29, 2014·Bulletin of Environmental Contamination and Toxicology·Shweta Singh, Ravi Kumar Asthana
Jun 1, 2014·Seminars in Liver Disease·Thomas J UrbanGuruprasad P Aithal
Jul 26, 2014·Ecotoxicology and Environmental Safety·Tammy A LeeJoshua J Faber-Hammond

❮ Previous
Next ❯

Citations

Jan 31, 2017·Toxicon : Official Journal of the International Society on Toxinology·Yiyi FengXiaoyu Li
Jan 22, 2019·Experimental Biology and Medicine·Paweł BrzuzanStefan Dobosz
Jun 9, 2018·Neurotoxicity Research·Maciej FlorczykMaciej Woźny
Nov 29, 2020·Molecular and Cellular Biochemistry·Zhi YangShipin Wu
May 1, 2020·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Jun GuoJian Li

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.