miR-122 promotes diabetic retinopathy through targeting TIMP3.

Animal Cells and Systems
Mingliang WangGuanghui Shao

Abstract

Diabetic retinopathy (DR) is a primary complication of diabetes mellitus. DR can cause severe vision loss for patients. miR-122 is elevated in DR patients, while its role in DR is unclear. Hence, the purpose of this study was to analyze the effect of miR-122 on the function of high glucose-induced REC cells and the underlying molecular mechanisms. In this study, our results revealed that miR-122 was up-regulated in high glucose-induced human retinal pigment epithelial cells (ARPE-19). High glucose decreased the cell viability of ARPE-19 cells, which was then restored by miR-122 knockdown. In addition, miR-122 knockdown suppressed apoptosis of high glucose-induced ARPE-19 cells. High glucose also inhibited B-cell lymphoma-2 (Bcl-2) level and increased cleaved caspase-3 level in ARPE-19 cells, which were reversed by miR-122 knockdown. Tissue inhibitor of metalloproteinases-3 (TIMP3) was a direct target of miR-122. TIMP3 was decreased in high glucose-induced ARPE-19 cells, and the decrease was abrogated by miR-122 knockdown. In addition, the effects of miR-122 overexpression in cell viability and apoptosis of high glucose-induced ARPE-19 were abolished by overexpression of TIMP3. In conclusion, the effect and mechanism of miR-122 ...Continue Reading

References

Jan 10, 2013·Investigative Ophthalmology & Visual Science·Nina Jasmin HewingCarl Peter Blobel
Mar 26, 2014·The British Journal of Ophthalmology·Rupert R A BourneUNKNOWN Vision Loss Expert Group of the Global Burden of Disease Study
Jan 6, 2015·Apoptosis : an International Journal on Programmed Cell Death·Jian Hua Qi, Bela Anand-Apte
Feb 7, 2016·The International Journal of Biochemistry & Cell Biology·Jin-Hui WuCui-Min Chen
Sep 15, 2016·World Journal of Diabetes·Michael W Stewart
Dec 29, 2017·Arteriosclerosis, Thrombosis, and Vascular Biology·Ann Marie Schmidt
May 10, 2018·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Dong-Mei WuYuan-Lin Zheng
Nov 28, 2018·Journal of Cellular Physiology·Rana ShafabakhshZatollah Asemi
Jan 24, 2019·Clinical and Experimental Medicine·Nina PastukhArnon Blum
Jul 11, 2019·Neural Regeneration Research·Bridget Martinez, Philip V Peplow

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
transfection

Software Mentioned

SPSS Statistics

Related Concepts

Related Feeds

B-Cell Lymphoma

B-cell lymphomas include lymphomas that affect B cells. This subtype of cancer accounts for over 80% of non-Hodgkin lymphomas in the US. Here is the latest research.