miR-1224-5p Enhances Hepatic Lipogenesis by Targeting Adenosine Monophosphate-Activated Protein Kinase α1 in Male Mice

Endocrinology
Tianxing ChenWu Yin

Abstract

MicroRNAs are potential therapeutic targets for metabolic diseases. Here, miR-1224-5p was highly expressed in the livers of mice fed a high-fat diet (HFD) and in obese (ob/ob) mice. To examine the potential role of miR-1224-5p, we constructed liver-specific adenoviral vectors expressing either an miR-1224-5p inhibitor sequence or miR-1224-5p mimic sequences. After tail-vein vector injection, HFD-fed mice were examined for expression of lipogenic genes. We found that miR-1224-5p inhibitors significantly attenuated hepatic lipogenesis and steatosis in HFD-fed mice, whereas miR-1224-5p mimicked promoted lipid accumulation in the liver of chow-fed C57BL/6 mice. Additional in vitro studies demonstrated that downregulation of miR-1224-5p in HepG2 and primary hepatocytes led to a reduction of cellular triglycerides after treatment with an oleic acid and palmitic acid mixture. Importantly, this study also identified adenosine monophosphate-activated protein kinase (AMPK)-α1 as a direct target of miR-1224-5p. miR-1224-5p binding to the 3' untranslated region of AMPKα1 suppressed expression of the AMPKα1 protein and its downstream molecules. Metformin, an activator of AMPK, also inhibited hepatic expression of miR-1224-5p. Together, thes...Continue Reading

References

Jul 16, 2003·Cold Spring Harbor Symposia on Quantitative Biology·J D HortonM S Brown
Dec 21, 2005·Biochemical and Biophysical Research Communications·Woo Je LeeJoong-Yeol Park
Aug 2, 2006·Annual Review of Pharmacology and Toxicology·D Grahame Hardie
Oct 24, 2008·Alimentary Pharmacology & Therapeutics·R LoombaJ H Hoofnagle
Feb 20, 2010·Current Opinion in Gastroenterology·Onpan Cheung, Arun J Sanyal
May 15, 2010·Science·S Hani Najafi-ShoushtariAnders M Näär
Nov 13, 2010·Arteriosclerosis, Thrombosis, and Vascular Biology·Liya YinYanqiao Zhang
Mar 9, 2011·Liver International : Official Journal of the International Association for the Study of the Liver·Quentin M Anstee
Mar 25, 2011·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Antero SalminenKai Kaarniranta
Mar 29, 2011·Clinical Gastroenterology and Hepatology : the Official Clinical Practice Journal of the American Gastroenterological Association·Zobair M YounossiManirath Srishord
Sep 29, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Ming HuMin You
Dec 17, 2011·International Journal of Radiation Oncology, Biology, Physics·Giuseppina Della Vittoria ScarpatiStefano Pepe
Jan 27, 2012·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Linkang ZhouPeng Li
Mar 23, 2012·Nature Reviews. Molecular Cell Biology·D Grahame HardieSimon A Hawley
Apr 17, 2012·Gastroenterology·Xin Wei WangHenrik Orum
Jun 22, 2012·Canadian Journal of Gastroenterology = Journal Canadien De Gastroenterologie·Melanie D Beaton
Jul 24, 2012·The Journal of Clinical Investigation·Shu-Hao HsuKalpana Ghoshal
Aug 2, 2012·Experimental Diabetes Research·Michael D Williams, Geraldine M Mitchell
Apr 30, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Ping MaXu Yang
May 30, 2013·Molecular and Cellular Biology·Cristina M RamírezCarlos Fernández-Hernando
Mar 29, 2014·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Raymond NgGuisheng Song
Jul 2, 2014·The Journal of Clinical Investigation·Sayaka Inokuchi-ShimizuEkihiro Seki
Sep 3, 2014·International Journal of Genomics·Ayla Valinezhad OrangMina Kazemzadeh-Bavili
Sep 10, 2014·Best Practice & Research. Clinical Gastroenterology·Peter Dietrich, Claus Hellerbrand
Feb 5, 2015·Molecular and Cellular Biochemistry·Jin QianYong-Ping You

❮ Previous
Next ❯

Citations

Jan 6, 2022·Journal of Cachexia, Sarcopenia and Muscle·Lisa DowlingKatarzyna Goljanek-Whysall

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.