miR-124-3p acts as a potential marker and suppresses tumor growth in gastric cancer

Biomedical Reports
Feng LiuLiming Xie

Abstract

miR-124-3p has been implicated in a variety of cancers. The purpose of the present study was to investigate the expression, prognostic roles and functions of miR-124-3p in gastric cancer. Functional studies indicated that ectopic overexpression of miR-124-3p in gastric cancer cells suppressed cell viability and plate colony formation in vitro and tumor growth in vivo. In situ hybridization analysis demonstrated that decreased expression of miR-124-3p was associated with clinical stage and lymph node metastasis, as well as shorter overall survival and disease-free survival rates. Furthermore, it was observed that miR-124-3p repressed the carcinogenesis of gastric cancer by targeting Ras-related C3 botulinum toxin substrate 1 (Rac1) and specificity protein 1 (SP1). Collectively, these results indicate a potential underlying mechanism for the regulation of gastric cancer by miR-124-3p involving targeting of Rac1 and SP1. Thus, miR-124-3p may be an independent indicator of survival and treatment strategy for patients with gastric cancer.

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Citations

Mar 21, 2019·American Journal of Physiology. Lung Cellular and Molecular Physiology·Qing-Chun LiZhen-Bo Su
Apr 21, 2021·Molecular Biology Reports·Qian LiFrederick T Greenaway

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Methods Mentioned

BETA
PCR
Assay
electrophoresis
transfection
xenograft
GTPase

Software Mentioned

SPSS
TargetScan

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