MiR-124-3p suppresses glioma aggressiveness via targeting of Fra-2

Pathology, Research and Practice
Lifei LuoJie Ling

Abstract

Malignant glioma is the most common and deadly primary brain tumor in adults. However, the mechanisms underlying the malignancy of glioma remain unclear. In the present study, we found that Fos-related antigen-2 (Fra-2) was overexpressed in most glioma cells, and knockdown of Fra-2 prevented cell proliferation, migration, and invasion. Mechanistically, Fra-2 silencing led to a significant reduction in cell-cycle drivers (Cyclin D1 and Cyclin E1), one invasion-associated gene (MMP9), the mesenchymal marker (Vimentin), and induction of the epithelial marker (E-cadherin). Further study confirmed that miR-124-3p decreased the expression of Fra-2 via directly targeting the 3'-UTR, and transfection with miR-124-3p in glioma cells inhibited expression of the above cell-cycle and EMT promoters. Phenotypic experiments also showed that overexpression of Fra-2 weakened the inhibitory effects of miR-124-3p on the proliferation, migration, and invasion of glioma cells. In addition, Fra-2 knockdown impaired the malignant phenotypes enhanced by miR-124-3p inhibition, which suggested a crucial role for the miR-124-3p/Fra-2 pathway in glioma development. Consistently, high expression of Fra-2 was closely associated with low miR-124-3p level and...Continue Reading

Citations

Aug 8, 2019·Cancer Medicine·Xinqi JiaShan Yu
Sep 26, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Junyi TanXintao Shuai
May 6, 2020·World Journal of Gastrointestinal Oncology·Chong-Bing SunXiao-Gang Leng
Oct 24, 2020·Frontiers in Molecular Biosciences·Abbasi MajidMing-Zhong Sun
May 24, 2021·Biochimica Et Biophysica Acta. Reviews on Cancer·Saleha AnwarMd Imtaiyaz Hassan
Sep 16, 2021·Drug Development Research·Hanxun YaoSheng Qiu

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