miR-125a induces apoptosis, metabolism disorder and migrationimpairment in pancreatic cancer cells by targeting Mfn2-related mitochondrial fission

International Journal of Oncology
Lichao PanRong Liu

Abstract

Mitochondrial fission is important for the development and progression of pancreatic cancer (PC). However, little is known regarding its role in pancreatic cancer apoptosis, metabolism and migration. In the current study, the mechanism by which mitochondrial fission modifies the biological characteristics of PC was explored. MicroRNA‑125a (miR‑125a) had the ability to inhibit mitochondrial fission and contributed to cellular survival. Suppressed mitochondrial fission led to a reduction in mitochondrial debris, preserved the mitochondrial membrane potential, inhibited mitochondrial permeability transition pore opening, ablated cytochrome c leakage into the cytoplasm and reduced the pro‑apoptotic protein contents, finally blocking mitochondria related apoptosis pathways. Furthermore, defective mitochondrial fission induced by miR‑125a enhanced mitochondria‑dependent energy metabolism by promoting activity of electron transport chain complexes. Furthermore, suppressed mitochondrial fission also contributed to PANC‑1 cell migration by preserving the F‑actin balance. Furthermore, mitofusin 2 (Mfn2), the key defender of mitochondrial fission, is involved in inhibition of miR125a‑mediated mitochondrial fission. Low contents of miR‑125...Continue Reading

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Jan 8, 2020·Cellular and Molecular Life Sciences : CMLS·Zhen TanChen Liang
Feb 1, 2019·Cancers·Marijke I ZonneveldKasper M A Rouschop
Sep 26, 2019·Frontiers in Endocrinology·Wenting Dai, Lei Jiang
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Jun 18, 2021·International Journal of Nanomedicine·Khaled S AllemailemAmjad Ali Khan
Aug 10, 2021·World Journal of Gastroenterology : WJG·Matias Estaras, Antonio Gonzalez

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Methods Mentioned

BETA
transfection
protein assay
PCR
Assay
flow cytometry
ELISA
GTPase

Software Mentioned

BD Paint - A - [UNK] Pro
Quantity One
SPSS

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