MiR-125b-5p is involved in oxygen and glucose deprivation injury in PC-12 cells via CBS/H2 S pathway

Nitric Oxide : Biology and Chemistry
Yaqi ShenYizhun Zhu

Abstract

Ischemic stroke is one of the leading causes of death worldwide. MicroRNAs (miRNAs) have been reported to be implicated in cerebral hypoxia injury and could serve as a therapeutic target. As the third gasotransmitter, hydrogen sulfide (H2S) plays a critical role in hypoxia-induced injury in the central nervous system. Cystathionine β-synthase (CBS) is the main enzyme catalyzing the production of H2S in brain. The objective of this study was to investigate the effect of miR-125b-5p on protecting against oxygen and glucose deprivation (OGD) injury in PC-12 cells by regulating CBS and H2S generation. The level of miR-125b-5p was increased in the rat MCAO model as well as OGD model in PC-12 cells. Meanwhile, CBS expression was remarkably downregulated. Overexpression of miR-125b-5p reduced CBS expression, decreased the H2S generation, and deteriorated OGD injury in PC-12 cells. On the contrary, silencing miR-125b-5p protected PC-12 cells from OGD injury by upregulated CBS and H2S levels. We found the protective effect of miR-125b-5p inhibition was associated with anti-oxidative and anti-apoptotic cell signaling through decreasing ROS level and reducing mitochondrial membrane potential (ΔΨm). Furthermore, the protective effect was a...Continue Reading

Citations

May 18, 2020·In Vitro Cellular & Developmental Biology. Animal·Ji-Lin FanShi-Liang Zhang
Nov 17, 2020·Oxidative Medicine and Cellular Longevity·Xiaoyu WangAnwen Shao
Jul 18, 2021·Journal of Stroke and Cerebrovascular Diseases : the Official Journal of National Stroke Association·Bayarmaa KhadankhuuYunman Li

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