PMID: 26770454Jan 16, 2016Paper

miR-125b suppresses the proliferation of hepatocellular carcinoma cells by targeting Sirtuin7

International Journal of Clinical and Experimental Medicine
Lixin Zhao, Weixing Wang

Abstract

Previous studies have shown that microRNAs are involved in many human cancers. However, the role of miR-125b in hepatocellular carcinoma (HCC) has not been fully understood. In our study, we detected the expression of miR-125b using Real-time quantitative-polymerase chain reaction (RT-PCR) and found that the expressions of miR-125b were significantly inhibited in HCC tissues and cell lines. The levels of miR-125b were associated with the degree of HCC malignancy. Otherwise, MTT assay showed that the proliferation was significantly decreased after transfection of miR-125b mimics into HepG2 cells; while the proliferation was significantly increased in HepG2 cells transfected with miR-125b inhibitors. Furthermore, TargetScan was conducted to predict the target gene of miR-125b and Sirtuin7 (SIRT7) was chosen to a potential target gene. And then we used luciferase reporter assay and western blot to confirm that SIRT7 is a direct target gene. Western blot indicated that transfection of miR-125b mimics could significantly inhibit the expression of SIRT7 in HepG2 cells, whereas, transfection of miR-125b inhibitor could significantly increase the expression of SIRT7 in HepG2 cells. These results suggest that miR-125b can inhibit the pr...Continue Reading

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