miR-128 Regulates Tumor Cell CD47 Expression and Promotes Anti-tumor Immunity in Pancreatic Cancer.

Frontiers in Immunology
Qing XiRongxin Zhang

Abstract

Pancreatic adenocarcinoma (PDAC) is a highly fatal disease worldwide. MicroRNAs (miRNAs) could regulate the protein-coding RNAs related to tumor growth, invasion, and immune evasion. Therefore, the investigation of novel miRNAs may be helpful in the development of more effective therapies for PDAC. In this study, we investigated the role and mechanism of action of miR-128 in PDAC. By using bioinformatics methods, we found that decreased expression of miR-128 was associated with poor overall survival of PDAC. miR-128 was inversely correlated with cluster of differentiation 47 (CD47), which was positively related to zinc finger E-box-binding homeobox 1 (ZEB1) in PDAC. Through in vivo experiments, we found that miR-128 could suppress the growth and metastasis of PDAC. Analysis of the immune microenvironment demonstrated that overexpression of miR-128 on tumor cells could increase the percentages of dendritic cells (DCs), CD8+ T lymphocytes, and natural killer T cells (NKT) in the tumor and spleen, consequently enhancing anti-tumor immunity. In vitro assays showed that miR-128 could inhibit cell proliferation, clonogenicity, migration, and invasion in Panc02 cells and could also enhance the phagocytosis of macrophages and the activ...Continue Reading

References

Apr 18, 2001·Trends in Cell Biology·E J Brown, W A Frazier
Oct 28, 2003·Nature Immunology·Manfred BriglMichael B Brenner
Dec 31, 2005·Nucleic Acids Research·Paul W C HsuIvo L Hofacker
Dec 8, 2006·Annual Review of Immunology·Albert BendelacLuc Teyton
Nov 18, 2008·Cellular and Molecular Life Sciences : CMLS·C VandewalleG Berx
Dec 6, 2008·Advances in Cancer Research·Masaki Terabe, Jay A Berzofsky
Aug 14, 2010·EMBO Reports·Simone Brabletz, Thomas Brabletz
Nov 2, 2011·Proceedings of the National Academy of Sciences of the United States of America·Xi Wen ZhaoTimo K van den Berg
Mar 28, 2012·Proceedings of the National Academy of Sciences of the United States of America·Stephen B WillinghamIrving L Weissman
Jan 22, 2013·Nature Reviews. Immunology·Patrick J BrennanMichael B Brenner
Jan 25, 2013·Nature Reviews. Cancer·Bram De Craene, Geert Berx
May 31, 2014·Nature Cell Biology·Alain PuisieuxJulie Caramel
Apr 4, 2015·Science·Padmanee Sharma, James P Allison
Apr 30, 2015·Japanese Journal of Clinical Oncology·Xianglun SunJie Zhang
Sep 1, 2015·International Journal of Oncology·Ramadevi SubramaniRajkumar Lakshmanaswamy
Sep 1, 2015·Nature Medicine·Xiaojuan LiuMeng Michelle Xu
Mar 2, 2016·International Journal of Biological Sciences·Yiwei Li, Fazlul H Sarkar
Apr 16, 2016·Nature Reviews. Cancer·Suzanne L TopalianDrew M Pardoll
May 10, 2016·Nature Reviews. Disease Primers·Jorg KleeffJohn P Neoptolemos
Jul 2, 2016·Cell·M Angela NietoJean Paul Thiery
Jan 6, 2017·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
Feb 18, 2017·Nature Reviews. Drug Discovery·Rajesha Rupaimoole, Frank J Slack
Mar 5, 2017·Immunological Reviews·Arlene H Sharpe
Mar 5, 2017·Immunological Reviews·Hanke L MatlungTimo K van den Berg
Mar 14, 2017·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Kipp Weiskopf
Nov 15, 2017·Nucleic Acids Research·Suhas V VasaikarBing Zhang
Dec 20, 2017·Cancer Research·Julie CaramelAlain Puisieux
Dec 31, 2017·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Alex D MichaelsTodd W Bauer
Jan 26, 2018·Molecular Cancer·Wei HuangYue-Qin Chen

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
density gradient centrifugation
FACS
flow cytometry
transfection

Software Mentioned

Image J
LinkedOmics
GraphPad Prism
FlowJo

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Related Papers

The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology
Sukanya ShyamasundarBoon Huat Bay
Experimental and Therapeutic Medicine
Hongchao HanAikun Wang
Experimental and Therapeutic Medicine
Jie YinShu Wang
© 2022 Meta ULC. All rights reserved