miR-130 aggravates acute myocardial infarction-induced myocardial injury by targeting PPAR-γ

Journal of Cellular Biochemistry
Xianglin ChuXiaofeng Chen

Abstract

Cardiac remodeling is a common pathophysiological change associated with acute myocardial infarction (AMI). Recent evidence indicates that microRNAs are strong posttranscriptional regulators which play an important role in regulating the microenvironment of myocardial tissue after AMI. In this study, we sought to explore the potential role and underlying mechanism of miR-130 in AMI. H9c2 cells were cultured under hypoxic conditions to simulate myocardial infarction. The influence of aberrantly expressed miR-130 on H9c2 cells under hypoxia was also estimated with RT-PCR, western blot and enzyme-linked immunosorbent assay. Using bioinformatics methods, of miR-130 target genes were verified with luciferase reporter assay. Then, the effects of miR-130 on AMI were identified in an induced myocardial injury model in rats. The results show that miR-130 downregulation remarkably decreased hypoxia-induced inflammation and fibrosis related protein expression in H9c2 cells and reversed hypoxia-induced peroxisome proliferator-activated receptor γ (PPAR-γ) inhibition. A bifluorescein reporter assay further confirmed that PPAR-γ was a target gene of miR-130. This study verified that PPAR-γ has a cardioprotective effect by inhibiting NFκB-med...Continue Reading

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Citations

Mar 7, 2020·Basic Research in Cardiology·Amela JusicUNKNOWN EU-CardioRNA COST Action (CA17129)
Oct 7, 2020·Pharmacology & Therapeutics·Qirong LuMaría-Aránzazu Martínez
Dec 17, 2020·International Journal of Molecular Sciences·Kay-Dietrich Wagner, Nicole Wagner
Apr 8, 2021·Scientific Reports·Maria Calderon-DominguezRocio Toro
Aug 8, 2021·International Journal of Molecular Sciences·Tong LiJiaqiang Huang
Oct 29, 2021·Frontiers in Veterinary Science·Woong-Bin RoHee-Myung Park
Oct 4, 2021·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Gordon Chun-Kau ChanCheuk-Chun Szeto
Jan 7, 2022·Journal of Cellular and Molecular Medicine·Meibin WangXiaoying Huang

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