MiR-130a is aberrantly overexpressed in adult acute myeloid leukemia with t(8;21) and its suppression induces AML cell death

Upsala Journal of Medical Sciences
Chao DingHai-Ping Dai

Abstract

Emerging evidence has revealed that miRNAs can function as oncogenes or tumor suppressor genes in leukemia. The ectopic expression of miR-130a has been reported in chronic leukemia, but our understanding of the biological implications of miR-130a expression remains incomplete. We quantified a cohort of de novo acute myeloid leukemia (AML) by bead-based miRNA and real-time quantitative PCR (Rq-PCR). The luciferase reporter gene assay was analyzed after the plasmid constructs which contain 5'-UTR of miR-130a and a Renilla luciferase reporter plasmid were transfected simultaneously into 293T cells. MTT and caspase 3/7 apoptosis assays were used to test cell viability and apoptosis. We identified miR-130a as significantly overexpressed in t(8;21) AML. Expression of miR-130a decreased significantly once patients with t(8;21) achieved complete remission, but increased sharply at the time of relapse. In patients with t(8;21) AML, KIT mutational status was associated with miR-130a expression-with higher expression associated with KIT activating mutations. Increased miR-130a expression in t(8;21) AML was associated with slightly worse event-free survival; however, no impact on overall survival was observed. Knockdown of AML1/ETO protein...Continue Reading

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Citations

Dec 6, 2018·Journal of Cellular Physiology·Rajab MardaniHossein Pourghadamyari
Oct 9, 2019·Cancer Biomarkers : Section a of Disease Markers·Zhigang FangJiajun Liu
Jul 14, 2020·Current Opinion in Oncology·Zhen HanChristiane Querfeld

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Methods Mentioned

BETA
transfection
PCR
Assay
ELISA

Software Mentioned

Excel
SPSS
CLUSTER

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