miR-133 involves in lung adenocarcinoma cell metastasis by targeting FLOT2

Artificial Cells, Nanomedicine, and Biotechnology
Guangxia WeiJie Yan

Abstract

Dysregulated microRNAs (miRNAs) reported to involve into the oncogenesis and progression in various human cancers. However, the roles and mechanism of miR-133 in lung adenocarcinoma remain largely unclear. In this study, qPCR assay and western blot were used to detect the expression levels of miR-133, Akt and FLOT2. Luciferase reporter assay was used to identify the target role of miR-133 on FLOT2. The cell invasion and the migration capability were performed using the transwell invasion assay and wound healing assay. We found that miR-133 expression levels were downregulated in human lung adenocarcinoma specimens and cell lines compared with the adjacent normal tissues and normal human bronchial epithelial cell. miR-133 significantly suppressed metastasis of lung adenocarcinoma cells in vitro. Furthermore, FLOT2 (flotillin-2) identified as a direct target of miR-133, and FLOT2 expression levels were inversely correlated with miR-133 expression levels in human lung adenocarcinoma specimens. And the restoration studies suggested FGF2 as a downstream effector of miR-133 which acted through Akt signalling pathway. Our study revealed the mechanism that miR-133 suppresses lung adenocarcinoma metastasis by targeting FLOT2 via Akt sig...Continue Reading

References

Mar 11, 2004·JAMA : the Journal of the American Medical Association·Ali H MokdadJulie L Gerberding
May 8, 2007·European Journal of Cell Biology·Tanja Babuke, Ritva Tikkanen
May 29, 2009·CA: a Cancer Journal for Clinicians·Ahmedin JemalMichael J Thun
Nov 8, 2011·Clinics in Chest Medicine·Scott Gettinger, Thomas Lynch
Aug 16, 2013·Journal of Translational Medicine·Xi WangHuan-Xin Lin
Nov 28, 2013·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Xu YangKe Lei
Feb 1, 2014·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Zheng LiJia Yu
Jul 6, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Jian-Qiang XuYao-Qi Yang
Jul 24, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yang YanJun-Hua Zheng
Aug 26, 2014·Cellular Signalling·Zhenguo ChengFeng Li
Oct 4, 2014·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Li WanZhaoxia Wang
Nov 19, 2014·Journal of Cancer Research and Clinical Oncology·Rui LiuJianda Zhou
Nov 27, 2015·Pathology Oncology Research : POR·Xiaojuan WuRong Ma

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Citations

Aug 27, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Yuqiang WangYaJun Miao

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