MiR-133a delivery to osteoblasts ameliorates mechanical unloading-triggered osteopenia progression in vitro and in vivo.

International Immunopharmacology
Youlong ZhouShuyun Ma

Abstract

Mechanical unloading-induced bone loss is a clinical challenge, and deep understanding for this disease is necessary for developing novel and effective therapies. MicroRNAs (miRNAs) are small non-coding RNAs, and involved in bone remodeling. In the study, we attempted to explore the potential of miR-133a in regulating osteoblast activation and its anti-osteopenia function both in vitro and in vivo. Our in vitro studies at first showed that miR-133a could significantly promote the expression of osteocalcin (OCN), Collagen I, alkaline phosphatase (ALP), runt-related transcription factor 2 (Runx2) and osterix (Osx), promoting the activation and mineralization of osteoblasts. Then, hindlimb unloading (HU)-challenged mice were established with or without intravenous injection of agomir-miR-133a using an osteoblast-targeting delivery system. We found that miR-133a in osteoblasts significantly alleviated the bone loss, microstructural, and biomechanical property in mice with mechanical unloading, contributing to osteopenia alleviation. Furthermore, both in vitro and in vivo experiments showed that miR-133a could restrain osteoclastogenesis via tartrate-resistant acid phosphatase (TRAP) staining. In conclusion, our results suggested th...Continue Reading

References

Dec 8, 2004·The Journal of Cell Biology·David M ThomasPhilip W Hinds
Nov 21, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Brenda J OlivierSchalk W van der Merwe
Jun 25, 2008·Journal of Prosthodontics : Official Journal of the American College of Prosthodontists·Jessica BoehrsGalen B Schneider
Mar 5, 2009·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Arata NakajimaRoland Baron
May 8, 2009·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Julio E Sotillo RodriguezRajaram Gopalakrishnan
May 5, 2011·Matrix Biology : Journal of the International Society for Matrix Biology·Takayuki HayamiSunil Kapila
May 12, 2011·PloS One·John R HawseThomas C Spelsberg
Dec 12, 2012·Nature Medicine·Xiaogang WangYingxian Li
Dec 25, 2012·The International Journal of Biochemistry & Cell Biology·Raghu BhushanPetra Knaus
Jan 17, 2013·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Hitomi NakamuraEijiro Jimi
Jun 27, 2013·Endocrinology·Xiao-Bo LiaoLing-Qing Yuan
Sep 24, 2013·Best Practice & Research. Clinical Endocrinology & Metabolism·Michaël LaurentDirk Vanderschueren
Aug 8, 2015·BioMed Research International·Rinaldo Florencio-SilvaPaulo Sérgio Cerri
Jan 31, 2017·Journal of Biological Physics·Charles DeckardBrent J F Hill
Apr 6, 2017·Bone Reports·Liam WilliamsonJonathan H Gooi
Jul 19, 2017·Journal of Exercise Nutrition & Biochemistry·Yun-Seok KangJae-Cheol Kim
Jan 23, 2018·Histochemistry and Cell Biology·Toshihisa Komori
Jul 10, 2018·Redox Biology·Suvi M KuosmanenAnna-Liisa Levonen
Jul 28, 2018·Joints·Ibrahim Akkawi, Hassan Zmerly
Feb 16, 2019·Molecular Therapy. Nucleic Acids·Naseer AhmadRitu Trivedi
Apr 12, 2019·Scientific Reports·Jitendra Prasad, Ajay Goyal
Apr 25, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Xinle LiPing Zhang
Nov 24, 2019·Biochemical and Biophysical Research Communications·Lijun ZhangShu Zhang
Dec 25, 2019·Environmental Science and Pollution Research International·Zhixing LiJiakui Li
Mar 24, 2020·Journal of Cachexia, Sarcopenia and Muscle·Ben KirkGustavo Duque
Sep 6, 2020·International Journal of Molecular Sciences·Anna-Jasmina DonaubauerUdo S Gaipl
Sep 18, 2020·Biological Research·Yang YangWang Shengwang
Oct 30, 2020·Viruses·Enakshi RoySt Patrick Reid

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.