MiR-133a is a potential target for arterial calcification in patients with end-stage renal disease.

International Urology and Nephrology
Sha LiYihao Mo

Abstract

Arterial calcification is an important risk factor for patients with end-stage renal disease. Despite substantial research efforts, the detailed mechanisms of the process of arterial calcification in end-stage renal disease remain unclear. miR-133a expression in radial artery samples was detected by FISH and Alizarin Red Staining. The expressions of miR-133a and RUNX2 in A7r5 cells with BMP2 induction were detected by qRT-PCR. In addition, qRT-PCR, Western blot, and ELISA assay were performed to detect changes in miR-133a levels in A7R5 cells after different treatments. Alizarin Red staining showed that red crystal deposition occurred in the tunica media. FISH analysis indicated that miR-133a was upregulated in the tunica media of the radial artery samples without calcification when compared with those with calcification. We also found that expression of RUNX2 in A7r5 cells increased at day 7 and day 14 after BMP2 induction and decreased miR-133a expression decreased at day 14. In addition, RUNX2 protein and OCN expression were upregulated in A7r5 cells during BMP2-induced calcification. When miR-133a expression was suppressed, cell calcification aggravated, which led to upregulation of RUNX2 and OCN. When miR-133a was overexpr...Continue Reading

References

Sep 17, 2004·Nature·Victor Ambros
May 1, 2007·Nature Medicine·Alessandra CarèGianluigi Condorelli
Jan 17, 2008·Nature Reviews. Genetics·Witold FilipowiczNahum Sonenberg
Feb 14, 2008·Nature Reviews. Molecular Cell Biology·Giovanni Stefani, Frank J Slack
Sep 12, 2008·Proceedings of the National Academy of Sciences of the United States of America·Zhaoyong LiGary S Stein
Dec 9, 2008·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Marguérite E I SchipperRoel A de Weger
Aug 1, 2009·American Journal of Respiratory and Critical Care Medicine·Yoshihiko ChibaMiwa Misawa
May 21, 2010·Physiological Genomics·Christine VoellenkleFabio Martelli
Jul 3, 2010·Circulation Research·Stephan FichtlschererStefanie Dimmeler
Sep 11, 2010·Clinical Journal of the American Society of Nephrology : CJASN·Francis VerbekeUNKNOWN CORD Study Investigators
Mar 30, 2011·Proceedings of the National Academy of Sciences of the United States of America·Tilde EskildsenMoustapha Kassem
Sep 3, 2011·Circulation Research·Catherine M ShanahanCecilia M Giachelli
Sep 7, 2011·Biochemical and Biophysical Research Communications·Jun LiuYi-Chun Zhu
Oct 11, 2011·Journal of Dental Research·W YangJ Gluhak-Heinrich
Mar 31, 2012·Circulation Research·Thomas Boettger, Thomas Braun
Apr 5, 2012·Nature Reviews. Endocrinology·Bithika Thompson, Dwight A Towler
Jun 27, 2013·Endocrinology·Xiao-Bo LiaoLing-Qing Yuan
Jul 17, 2014·Nature Reviews. Molecular Cell Biology·Minju Ha, V Narry Kim
Jul 25, 2015·International Journal of Oral Science·Dian JingZhi-He Zhao
Dec 29, 2015·Developmental Biology·Martin HorakJulie Bienertova-Vasku
Jan 6, 2016·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Vishal Agrawal, Mukty Sinha
Aug 1, 2013·Journal of Biomedical Science and Engineering·Maureen BeedermanTong-Chuan He
Sep 27, 2016·Medical Science Monitor Basic Research·Arkady RutkovskiyIngvar Jarle Vaage
Dec 10, 2016·Journal of the American College of Cardiology·Temo BarwariManuel Mayr
Feb 2, 2018·International Journal of Molecular Sciences·Shufang Zhang, Ning Chen
Mar 8, 2018·Cardiovascular Research·Andrew L DurhamCatherine M Shanahan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.