miR-136 Inhibits Malignant Progression of Hepatocellular Carcinoma Cells by Targeting Cyclooxygenase 2

Oncology Research
Haiyan JiaPibao Li

Abstract

MicroRNAs (miRNAs) play a vital role in regulating tumor progression. Dysregulated miR-136 expression was linked to the development of various human cancers. In the present study, we investigated the expression and relationship of miR-136 and COX2 in hepatocellular carcinoma (HCC) using relevant experiments, involving CCK-8, Transwell assay, and luciferase reporter assay. We demonstrated that miR-136 expression is obviously decreased in HCC tissues and cells, and negatively correlated with the expression of COX2 mRNA. In vitro assay revealed that overexpression of miR-136 significantly changed the expression of proliferation- and metastasis-related proteins and inhibited the proliferation, migration, and invasion of HepG2 and Hep3B cells. Dual-luciferase reporter assay validated that the 3'-untranslated region (3'-UTR) of COX2 is a direct target of miR-136. Furthermore, COX2 siRNA partially enhanced the miR-136 overexpression-induced inhibitory effects. In conclusion, miR-136 was vital in the regulation of HCC cell proliferation and metastasis by targeting COX2. Thus, our findings provided novel evidence that miR-136 might be recommended as a potential target for the diagnosis and treatment of HCC patients.

Citations

Aug 11, 2018·Cancer Metastasis Reviews·Dali TongJun Jiang
Dec 19, 2019·Cancer Metastasis Reviews·Karina Bezerra SalomãoMaría Sol Brassesco

Related Concepts

Prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase), human
MIRN136 microRNA, human
Cell Motility
Liver Carcinoma
Malignant Neoplasm of Liver
Tumor Cells, Cultured
Tumor Markers
Gene Expression Regulation, Neoplastic
Apoptosis, Intrinsic Pathway
Disease Progression

Related Feeds

Carcinoma, Hepatocellular

Hepatocellular Carcinoma is a malignant cancer in liver epithelial cells. Discover the latest research on Hepatocellular Carcinoma here.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.

Related Papers

Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie
Junjie QuanLe Zhou
Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
Hui LiuXueying Long
© 2021 Meta ULC. All rights reserved