miR-138-5p inhibits the malignant progression of prostate cancer by targeting FOXC1

Cancer Cell International
Dapeng ZhangXin Chen

Abstract

This study aimed to uncover the effect of miR-138-5p on the proliferation and metastasis of PCa cell lines, and further explore the potential regulatory mechanisms via regulating FOXC1. 60 pairs cancer tissues and corresponding paracancerous ones from PCa patients were collected to assess the expression level of miR-138-5p by qRT-PCR. Subsequently, over-expression of miR-138-5p were established to explore the proliferation and metastasis of miR-138-5p in PCa cell lines was analyzed by CCK-8, Transwell assay and Wounding healing assay, respectively. Bioinformatics analysis and luciferase reporter gene assay were performed to search for the target genes of miR-138-5p, and FOXC1 was selected. Finally, the biological role of miR-138-5p and FOXC1 in the progression of PCa was clarified by a series of rescue experiments. The results of qRT-PCR revealed that miR-138-5p was lowly expressed in PCa tissues and cell lines. Besides, the PCa patients with low-miR-138-5p had a high Gleason score, lymph node metastasis and poor prognosis of PCa, compared with these patients with high-miR-138-5p. Over-expression of miR-138-5p inhibited the proliferative, migratory and invasive capacities of PC-3 and DU-145 cells. Bioinformatics analysis and lu...Continue Reading

References

Nov 24, 2012·Briefings in Bioinformatics·Bing LiuMurray J Cairns
Nov 26, 2013·Bioinformatics·Søren MørkLars Juhl Jensen
Dec 4, 2013·Nature Reviews. Urology·Rosalind EelesZsofia Kote-Jarai
Apr 15, 2014·The Urologic Clinics of North America·Richard J Bryant, Hans Lilja
May 13, 2014·Internal Medicine Journal·C PezaroI D Davis
May 17, 2014·Asian Journal of Andrology·Min QuYing-Hao Sun
Sep 6, 2014·Current Pharmaceutical Biotechnology·Yusuf Tutar
Jan 12, 2016·Clinical Chemistry and Laboratory Medicine : CCLM·Xavier Filella, Laura Foj
Jun 2, 2016·Oncotarget·Lian ZhaoShourong Shen
Aug 30, 2016·MicroRNA·Wen-Juan Ni, Xiao-Min Leng
Dec 14, 2016·Nature Reviews. Urology·Annette Fenner
Feb 18, 2017·Nature Reviews. Drug Discovery·Rajesha Rupaimoole, Frank J Slack
Feb 24, 2017·Journal of Inherited Metabolic Disease·Ana Rivera-BarahonaLourdes R Desviat
Mar 23, 2017·BMC Urology·Hung N LuuGiuliano Di Pietro
Apr 28, 2017·Biomolecular Concepts·Virginie Armand-Labit, Anne Pradines
May 30, 2017·The Journal of Urology·Daniel J LeeDaniel A Barocas
Sep 7, 2017·BMC Cancer·Ivan E FloresKristin L Gosselink
Feb 17, 2018·Therapeutic Advances in Medical Oncology·Zhi YangYang Yang
Aug 28, 2018·MicroRNA·Majid Tafrihi, Elham Hasheminasab
Sep 2, 2018·Environmental Geochemistry and Health·Minghui LiXijin Xu
Sep 20, 2018·Advances in Experimental Medicine and Biology·Heide Schatten
Nov 13, 2019·Clinical Chemistry and Laboratory Medicine : CCLM·Michael J Duffy
Nov 23, 2019·Cells·Christiaan J Stavast, Stefan J Erkeland
Nov 26, 2019·Biochemistry. Biokhimii︠a︡·E V KalininaN N Chernov

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Citations

Dec 11, 2020·BioMed Research International·Yuting XuFang Zheng
Oct 6, 2021·International Journal of Immunopathology and Pharmacology·Ke LiLi Zhuang

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Methods Mentioned

BETA
transfection
PCR
Protein Assay
xenograft
transfect
xenografts

Software Mentioned

TargetScan
alpha SP

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