MiR-140/BDNF axis regulates normal human astrocyte proliferation and LPS-induced IL-6 and TNF-α secretion

Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie
Zhiming TuBing Wang

Abstract

Reactive astrocyte proliferation after spinal cord injury (SCI) contributes to glial scar formation that impedes axonal regeneration. The mechanisms underlying astrocyte proliferation upon injury remain partially understood. MicroRNAs (miRNAs) function as a major class of post-transcriptional gene expression regulators that participate in many biological processes. In this study, we focused on the functional role of miR-140 in normal human astrocyte (NHA) cell proliferation. Ectopic miR-140 expression significantly inhibited NHA cell viability and proliferation; miR-140 inhibition exerted the opposite function. Commonly, miRNAs exert functions through targeting downstream genes to inhibit their expression. In the present study, brain-derived neurotrophic factor (BDNF), a regulator of astrocyte proliferation and differentiation, confirmed as a direct target of miR-140 in NHA. Through binding to the 3'UTR of BDNF, miR-140 inhibited BDNF expression. BDNF overexpression significantly promoted NHA cell viability and proliferation; the regulatory effect of miR-140/BDNF on NHA proliferation was mediated by PI3K/AKT pathway. Moreover, we evaluated the functional role of miR-140 in Lipopolysaccharide (LPS)-induced in vitro injury model ...Continue Reading

Citations

Dec 7, 2018·Journal of Neuroinflammation·Vito Dozio, Jean-Charles Sanchez
Sep 15, 2020·Journal of Receptor and Signal Transduction Research·Jiajia LiYinghui Wu
Dec 15, 2018·Glia·Qiao-Qiao Yang, Jia-Wei Zhou
Jun 25, 2019·Frontiers in Neurology·Yang JinSong Bai Xu
Jan 9, 2019·Frontiers in Molecular Neuroscience·Kristen T ThomasGary J Bassell
Sep 5, 2020·Oxidative Medicine and Cellular Longevity·Xinyu LiBin Ning
Feb 14, 2020·International Journal of Molecular Sciences·Ewelina PalaszGrazyna Niewiadomska
Jun 11, 2020·Life·Federico MucciLiliana Dell'Osso
Nov 22, 2019·Computational Biology and Chemistry·Fatemeh Khani-HabibabadiMehrdad Behmanesh
May 7, 2021·Regenerative Medicine·Xu-Dong GuoXue-Wen Kang

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