Jan 7, 2019

MiR-141-3p functions as a tumor suppressor through directly targeting ZFR in non-small cell lung cancer

Biochemical and Biophysical Research Communications
Weisong LiLiantang Wang

Abstract

MicroRNAs are important regulators in the development and progression of non-small cell lung cancer (NSCLC). MiR-141-3p has been reported to function as a suppressor or oncogene in several tumors, but the clinical significance and crucial biological functions of miR-141-3p in NSCLC remains largely unclear. In this study, expression levels of miR-141-3p in tissue samples were measured by quantitative real-time polymerase chain reaction. Kaplan-Meier survival analysis and Cox regression assay were performed to evaluate the prognostic value of miR-141-3p. Cell experiments, including CCK-8, colony formation and transwell assays were carried out to explore its functional role. Luciferase reporter assay was used to confirm its target gene. The results showed that miR-141-3p was significantly down-regulated in NSCLC tissues compared with adjacent normal tissues. The decreased miR-141-3p expression was associated with advanced TNM stage and lymph-node metastasis. Patients with low miR-141-3p expression had poor overall survival compared with those with high expression. Down-regulation of miR-141-3p was demonstrated to be an independent prognostic factor for NSCLC. Moreover, ZFR was confirmed as a target gene of miR-141-3p. Meta-analysi...Continue Reading

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Mentioned in this Paper

Study
Gene Expression Regulation, Neoplastic
Tumor Suppressor Genes
Meta Analysis (Statistical Procedure)
TNM Staging System
MIR141
Genes
Cell Motility
Metastasis Suppressor Genes
Neoplasms

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