May 13, 2019

MiR-141-3p inhibits cell proliferation, migration and invasion by targeting TRAF5 in colorectal cancer

Biochemical and Biophysical Research Communications
Zhi LiangJiaotao Xing

Abstract

Emerging evidence has shown that abnormal microRNA (miRNA) expression play an important role in initiation, progression and metastasis in several tumors, including colorectal cancer. Here, we attempted to explore the expression and function of miR-141-3p in colorectal cancer. MiR-141-3p expression was measured in tissue samples, colorectal cancer cell lines and normal human colon epithelium cell line FHC by real-time PCR. The biological roles of miR-141-3p in colorectal cancer were investigated both in vitro and a mouse model in vivo. Bioinformatics analysis, real-time PCR, Western blot and luciferase reporter analysis were performed to validate the association between miR-141-3p and its potential targets. Our results suggested that miR-141-3p expression was down-regulated in colorectal cancer tissues and colorectal cancer cell lines compared to the normal tissues and normal colon cells. Patients with low miR-141-3p had poor outcome. In addition, Overexpression of miR-141-3p significantly delayed the proliferation, migration, and invasion of colorectal cancer cells in vitro, as well as obviously attenuated tumor growth in a xenograft model in vivo. Furthermore, Our results showed that miR-141-3p inhibited the proliferation, mig...Continue Reading

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Mentioned in this Paper

TNF receptor-associated factor 5
Real-Time Polymerase Chain Reaction
In Vivo
Tumor Suppressor Genes
MIR141
Western Blotting
Tumor Necrosis Factor Receptor-Associated Factors
Neoplasms
Bio-Informatics
Xenograft Model

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