miR-141 Promotes Colon Cancer Cell Proliferation by Targeted PHLPP2 Expression Inhibitionn

Cancer Management and Research
Fazhuang FangHuizhong Zhang

Abstract

Colon cancer (CC) is the third most common cancer with a high rate of incidence and mortality. Therefore, it is highly necessary to explore novel targets of CC. The miRNA-seq and RNA-seq data of CC were accessed from the TCGA database. Differential analysis was performed using the "edgeR" package to identify differentially expressed miRNAs (DE_miRNAs). The downstream target genes of the target miRNA were then predicted by miRNA target prediction databases to identify the target mRNA. Normal colon cell line CCD-18Co and CC cell lines HCT-116, HT-29, SW620 and SW480 were chosen, and qRT-PCR was conducted to detect miR-141 expression in these cell lines. qRT-PCR and Western blot were carried out to determine PHLPP2 mRNA and protein expression, respectively. Dual-luciferase reporter gene assay was performed to verify the targeting relationship between miR-141 and PHLPP2 3'UTR. CCK-8 assay and colony formation assay were carried out to detect cell proliferation. Meanwhile, tumor xenograft model in nude mice was constructed to assess CC cell tumorigenic ability in vivo. miR-141 was markedly up-regulated in CC tissue. CC cell proliferation and in vivo tumorigenic ability were suppressed by miR-141 silencing but promoted by miR-141 ove...Continue Reading

References

Sep 17, 2004·Nature·Victor Ambros
Jul 10, 2007·Cancer Research·Kati P PorkkaTapio Visakorpi
Sep 19, 2007·Cancer Research·Marilena V IorioCarlo M Croce
Jul 28, 2009·Annual Review of Medicine·Ramiro GarzonCarlo M Croce
Mar 22, 2014·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Xuanyu ChenXiaoping Zhang
Feb 2, 2016·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Yanping GaoLongbang Chen
Feb 23, 2017·International Journal of Cancer. Journal International Du Cancer·Michaela StrotbekMonilola A Olayioye
Apr 24, 2017·European Journal of Pharmacology·Chang-Feng LiDan-Dan Li
Sep 18, 2018·Archives of Biochemistry and Biophysics·Jia-Heng LiZhen Li
Nov 19, 2018·Critical Reviews in Oncology/hematology·Julia B KrajewskaPaula Mosińska
May 31, 2019·The Journal of Cell Biology·Roxanne Toivanen, Luc Furic
Oct 23, 2019·Urologic Oncology·Rola SaleebGeorge M Yousef
Nov 23, 2019·Cancer Biotherapy & Radiopharmaceuticals·Ye JinLingli Yu
Nov 23, 2019·Reproduction : the Official Journal of the Society for the Study of Fertility·Hu GaoBin Chen

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Methods Mentioned

BETA
RNA-seq
transfection
PCR
Assay
electrophoresis
transfect
xenograft

Software Mentioned

ImageProPlus6
TargetScan
SPSS
edgeR

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