MiR-142-5p Protects Against 6-OHDA-Induced SH-SY5Y Cell Injury by Downregulating BECN1 and Autophagy

Dose-response : a Publication of International Hormesis Society
Jian ChenChun-Li Xie

Abstract

MiR-142-5p has been demonstrated to hold significant implications in neurological diseases. However, the impact and underlying regulatory mechanism of miR-142-5p in Parkinson's disease (PD) are still ominous. To simulate the PD, 6-hydroxydopamine (6-OHDA)-treated SH-SY5Y cell model was used in this study. Levels of messenger RNA and protein were tested by quantitative real-time polymerase chain reaction and Western blot analyses, respectively. The direct interaction between miR-142-5p and Beclin 1 (BECN1) was assessed by luciferase reporter assay. Furthermore, Cell Counting Kit-8 assay was performed to assess cytotoxicity of SH-SY5Y cell. In consequence, a significant decrease of miR-142-5p was observed in 6-OHDA-induced SH-SY5Y cells. Over-/Low-expressed miR-142-5p resulted in a significant enhancement/inhibition on cell vitalities of 6-OHDA-treated SH-SY5Y cells, which might be modulated by repressing cellular autophagy through inhibiting level of BECN1 and LC3 II/LC3 I and elevating P62 level. Luciferase reporter assay showed that the BECN1 was the target gene of miR-142-5p. Additionally, the loss/gain of BECN1 rescued/blocked the effects of miR-142-5p on the viability of 6-OHDA-induced SH-SY5Y cells. These results highlight...Continue Reading

References

Jun 3, 1999·Brain Research. Molecular Brain Research·A WoodgateM Dragunow
Mar 9, 2006·The Journal of Biological Chemistry·Mamoru ShibataJunying Yuan
May 26, 2007·The American Journal of Pathology·Yunhui ChengChunxiang Zhang
Sep 1, 2007·Science·Jongpil KimAsa Abeliovich
Jan 11, 2008·Neuron·Philip S ChoiCatherine Dulac
Jan 12, 2008·Brain : a Journal of Neurology·Tianhong PanJoseph Jankovic
Jan 15, 2008·Cell·Beth Levine, Guido Kroemer
Jul 25, 2009·Proceedings of the National Academy of Sciences of the United States of America·Eunsung JunnM Maral Mouradian
Oct 30, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Brian SpencerEliezer Masliah
Feb 1, 2011·Journal of Molecular Cell Biology·Liqin Du, Alexander Pertsemlidis
Mar 23, 2011·Expert Reviews in Molecular Medicine·Maria Xilouri, Leonidas Stefanis
Jun 8, 2012·Cold Spring Harbor Perspectives in Medicine·João Massano, Kailash P Bhatia
Mar 19, 2013·Behavioral and Brain Functions : BBF·Nian XiongTao Wang
Apr 5, 2013·Immunological Reviews·Esmerina TiliCarlo M Croce
Jun 12, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Amrita Datta ChaudhuriHoward S Fox
Mar 5, 2014·Proceedings of the National Academy of Sciences of the United States of America·Cheng QinZhiming Zhang
May 27, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Margherita GrassoMichela A Denti
Sep 16, 2014·Advanced Drug Delivery Reviews·Guofeng Cheng
Sep 19, 2014·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Doo Chul ChoiEunsung Junn
Aug 13, 2015·ELife·Vikram AgarwalDavid P Bartel
Apr 28, 2016·Progress in Neuro-psychopharmacology & Biological Psychiatry·Julia Hernandez-RappSébastien S Hébert
Aug 5, 2017·Frontiers in Molecular Neuroscience·Juhyun Song, Young-Kook Kim

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Methods Mentioned

BETA
transfection
PCRs
PCR
electrophoresis

Software Mentioned

GraphPad Prism
SPSS
TargetScan

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