MiR-143 and miR-135 inhibitors treatment induces skeletal myogenic differentiation of human adult dental pulp stem cells

Archives of Oral Biology
Dongxia LiYing Li

Abstract

Dental pulp stem cells (DPSCs) possess pluripotent properties that allow them to differentiate into multiple cell lineages, which can be potentially used in tissue regeneration. The aim of this in vitro study is to explore the effect of miRNAs on the myogenic differentiation of human adult DPSCs and seek for some potential biological factors for stable and feasible application in DPSC myogenic differentiation. Human adult DPSCs were isolated from normal impacted third molars were treated with 5-Aza-2'-deoxycytidine to induce to myogenic differentiation in vitro. During this process the levels of myomiRNAs and myogenic marker genes were detected by real-time qPCR and Western blotting. Then antisense oligonucleotides of miR-143 and miR-135 were transfected into DPSCs to explore their effects on myogenic differentiation. Gene expression detection and MyHC immunofluorescence microscopy analysis were applied to characterize the myogenic differentiation of DPSCs. Expression of miR-135 and miR-143 was markedly decreased in myoblast DPSCs induced by 5-Aza. Part of the DPSCs treated with miR-135 or miR-143 inhibitors showed apparent myocytic properties and eventually fused to form myotubes. Co-transfection of miR-135 and miR-143 inhibit...Continue Reading

References

Nov 23, 2000·Proceedings of the National Academy of Sciences of the United States of America·S GronthosS Shi
Jul 31, 2002·Journal of Dental Research·S GronthosS Shi
Apr 17, 2007·Tissue Engineering·You-Young JoPill-Hoon Choung
Apr 3, 2008·Biochimica Et Biophysica Acta·John J McCarthy
Apr 15, 2009·Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia·Jakub SuchanekJaroslav Mokry
Apr 7, 2010·Archives of Oral Biology·Ryusuke NakatsukaYoshiaki Sonoda
Sep 18, 2012·Archives of Oral Biology·Nobuyuki Kawashima

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Citations

Oct 12, 2016·Gene·Amer SehicTor Paaske Utheim
Sep 22, 2020·Journal of Cachexia, Sarcopenia and Muscle·Wei LvBo Zuo
Mar 20, 2019·Journal of Cellular Physiology·Mei LiuHong Chen
Apr 23, 2020·The Kaohsiung Journal of Medical Sciences·Xiang-Tao ZhangFei Wang
Dec 23, 2017·Frontiers in Physiology·Beatriz A Rodas-JuncoGeovanny I Nic-Can
Sep 28, 2018·Stem Cell Research & Therapy·Syed Shadab RazaMohsin Ali Khan
Oct 20, 2020·Frontiers in Physiology·Bernardo Bonilauri, Bruno Dallagiovanna
Nov 6, 2020·Stem Cells International·Dan ZhouWei Du

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