miR-144-3p, a tumor suppressive microRNA targeting ETS-1 in laryngeal squamous cell carcinoma

Oncotarget
Si-Yi ZhangYi-Long Wu

Abstract

Regional lymph node metastasis and distant metastasis are critical in the prognosis of laryngeal squamous cell carcinoma (LSCC). This study investigated the roles of miR-144-3p and E26 transformation specific-1 (ETS-1) in the invasion and migration of LSCC cells. The effects of miR-144-3p and ETS-1 on FaDu and Hep2 cell growth, migration and invasion were determined. Suppression of ETS-1 by miR-144-3p was confirmed using luciferase assays; the effects of ETS-1 silencing were determined using a xenograft tumor model. The expression of ETS-1 was analyzed in 71 paraffin-embedded tissue biopsies and eight fresh frozen biopsies obtained from LSCC patients. miR-144-3p inhibited the growth, invasion and migration of FaDu and Hep2 cells in part through suppression of epithelial-mesenchymal transition as determined by increased E-cadherin and α-catenin and reduced fibronectin and vimentin expression. Additionally, ETS-1 is a molecular target of miR-144-3p, and silencing ETS-1 expression inhibited FaDu and Hep2 cell invasion and migration as well as reduced Hep2 xenograft tumor volume. In LSCC, the expression of ETS-1 is upregulated with disease progression, and higher ETS-1 expression, which was negatively associated with miR-144-3p lev...Continue Reading

References

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Citations

Mar 5, 2016·Cell Cycle·María José de Miguel-LukenAmancio Carnero
Aug 12, 2017·Cancer Biomarkers : Section a of Disease Markers·Suoning LiuWei Li
Nov 19, 2019·Biotechnology and Applied Biochemistry·Xianjie ZhangYuKai Zhou
Jul 2, 2019·Experimental and Therapeutic Medicine·Ruoxi Yin, Xiaoya Chen
Feb 2, 2018·Experimental and Therapeutic Medicine·Pingping TaoQing Li
Oct 23, 2019·Scientific Reports·Berta Roman-CanalEva Colas
Jul 31, 2021·Computational and Mathematical Methods in Medicine·Bin-Yu MoSu-Ning Huang

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Methods Mentioned

BETA
transfection
Xenograft
biopsies
flow cytometry
PCR
electrophoresis

Software Mentioned

PicTar
TargetScan
miRanda
SPSS
Primer Express

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