miR-145-5p Inhibits Vascular Smooth Muscle Cells (VSMCs) Proliferation and Migration by Dysregulating the Transforming Growth Factor-b Signaling Cascade

Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
Li LiMing Li

Abstract

BACKGROUND There is accumulating evidence demonstrating that microRNAs (miRNA) play essential roles in proliferation, migration, and invasion of vascular smooth muscle cells (VSMCs). However, the exact function of these molecules and the mechanisms involved are not fully understood. In this study, we defined the role of miR-145-5p in VSMCs. MATERIAL AND METHODS This study used the PDGF-bb-induced VSMCs proliferation model. Expression of miR-145-5p and its target, Smad4, were detected and measured by real-time PCR and Western blot analysis. The luciferase reporter of miR-145-5p was used to elucidate miRNA-target interactions. The functions of miR-145-5p in proliferation and migration were detected by CCK-8 assay, Transwell assay, and scratch test. RESULTS This study demonstrates that miR-145-5p is downregulated in PDGF-mediated VSMCs in both time- and dose-dependent manners. The in vitro results suggest that overexpression of miR-145-5p results in a reduction in SMAD4 and an increase in SMAD2, Smad3, and TGF-β at the mRNA and protein levels. Overexpression of miR-145-5p inhibited PDGF-induced VSMCs proliferation and migration. Moreover, SMAD4 was identified as a direct target of miR-145-5p and is involved in PDGF-mediated VSMC p...Continue Reading

Citations

Jan 17, 2019·International Journal of Molecular Sciences·Dongdong Wang, Atanas G Atanasov
Nov 14, 2018·Menopause : the Journal of the North American Menopause Society·Sung Hwan ChoNam Keun Kim
Dec 21, 2019·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·Zheng YangMin Qiu
Nov 17, 2020·Cancer Management and Research·Ke WangYi-Min Wang
Feb 23, 2021·Biochemical Society Transactions·Kirsten Riches-Suman
Jul 20, 2021·Bioinformatics and Biology Insights·Romana IshratArmiya Sultan
Aug 24, 2021·Investigative Ophthalmology & Visual Science·Wanju YangYiqiao Xing

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Methods Mentioned

BETA
transfection
PCR

Software Mentioned

TargetScan
ImageJ
miRanda

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