MiR-145 reduces the activity of PI3K/Akt and MAPK signaling pathways and inhibits adipogenesis in bovine preadipocytes.

Genomics
Li WangLinsen Zan

Abstract

Adipose tissue is the largest metabolic organ because of adipogenesis controlled by numerous miRNAs. MiR-145 is classified into the same cluster with famous miR-143. However, few studies have investigated the role of miR-145 in adipogenesis. In the current study, we observed that the expression of miR-145 was downregulated during bovine adipogenesis in vivo and in vitro. The results of RNA-Seq analysis showed that miR-145 mainly disturb the PI3K/Akt and MAPK signaling pathways in bovine preadipocytes. MiR-145 inhibited bovine preadipocyte differentiation and downregulated phosphorylation level of Akt and ERK1/2 proteins. Furthermore, insulin, as a powerful inducer initiating adipogenesis and an activator of the PI3K/Akt and MAPK signaling pathways, was able to rescue the downregulation of Akt and ERK1/2 phosphorylation levels caused by miR-145. Taken together, our findings suggest that miR-145 is a potent inhibitor of adipogenesis that may function by reducing the activity of PI3K/Akt and MAPK signaling pathways.

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Citations

Sep 2, 2020·International Journal of Molecular Sciences·Sara CrucianiMargherita Maioli
Feb 27, 2021·Frontiers in Genetics·Xingyi ChenLinsen Zan
Mar 18, 2021·Molecular and Cellular Biochemistry·Zhaoxiong LeiYun Ma
Apr 4, 2021·Animals : an Open Access Journal From MDPI·Xiayang JinYuzhu Luo
May 1, 2021·Animals : an Open Access Journal From MDPI·Hongfang GuoLinsen Zan
Jul 11, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Nahid AlimoradiReihaneh Fatehi

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