miR-146a is involved in the regulation of vertebrate LC-PUFA biosynthesis by targeting elovl5 as demonstrated in rabbitfish Siganus canaliculatus

Gene
Cuiying ChenYuanyou Li

Abstract

Rabbitfish Siganus canaliculatus is the first marine teleost demonstrated to have the ability to synthesize long-chain polyunsaturated fatty acids (LC-PUFA) from C18 PUFA precursors, and thus provides us a unique model for studying the regulatory mechanisms of LC-PUFA biosynthesis in teleosts. MicroRNAs (miRNAs) were shown to play important roles in the regulation of LC-PUFA biosynthesis in rabbitfish at posttranscriptional level in our previous studies. Here, we focused the roles of miR-146a in such regulation. The expression of miR-146a displayed an inverse pattern with that of elongase 5 (Elovl5), a key enzyme catalyzing the elongation of C18 (18:4n3 and 18:3n6) and C20 (20:5n3 and 20:4n6) PUFA in the LC-PUFA biosynthesis, in vivo in liver of rabbitfish reared under different salinities, as well as in vitro in S. canaliculatus hepatocyte line (SCHL) cells incubated with different fatty acids. Bioinformatics analysis predicted that miR-146a may target the 3'UTR of elovl5 directly, which was confirmed by the dual luciferase reporter assays in HEK 293T cells. Overexpression of miR-146a significantly downregulated the expression of elovl5 in SCHL cells, while knockdown of miR-146a showed an opposite effect. Moreover, up-regulati...Continue Reading

References

Jan 1, 1989·Journal of Internal Medicine. Supplement·A Nordøy, J Dyerberg
Nov 28, 1980·Science·F A Kuehl, R W Egan
Feb 15, 2002·Current Opinion in Clinical Nutrition and Metabolic Care·J Thomas Brenna
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
May 15, 2002·Current Biology : CB·Mariana Lagos-QuintanaThomas Tuschl
Jan 23, 2003·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Rodolfo R Brenner
Jun 24, 2004·Nature Reviews. Genetics·Lin He, Gregory J Hannon
Sep 17, 2004·Nature·Victor Ambros
Nov 1, 2005·Nature·Jan KrützfeldtMarkus Stoffel
Mar 4, 2006·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Giovanni M TurchiniSena S De Silva
Mar 28, 2006·Progress in Lipid Research·Andreas JakobssonAnders Jacobsson
Aug 4, 2006·Proceedings of the National Academy of Sciences of the United States of America·Konstantin D TaganovDavid Baltimore
Jul 22, 2008·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Yuan-You LiJun-Hui Huang
Oct 29, 2008·Genome Research·Robin C FriedmanDavid P Bartel
Jan 13, 2009·Biochimica Et Biophysica Acta·Yan QinHilde I Nebb
Jun 9, 2009·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Robert S ChapkinDavid N McMurray
Oct 6, 2009·Biochemical and Biophysical Research Communications·Michael KarbienerMarcel Scheideler
Dec 19, 2009·Progress in Lipid Research·Hervé GuillouAnders Jacobsson
Jan 12, 2010·Biochemical and Biophysical Research Communications·Sang Yun KimJae Bum Kim
May 15, 2010·Science·S Hani Najafi-ShoushtariAnders M Näär
May 15, 2010·Science·Katey J RaynerCarlos Fernández-Hernando
Jun 23, 2010·Proceedings of the National Academy of Sciences of the United States of America·Tyler J MarquartAngel Baldán
Sep 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Yuanyou LiDouglas R Tocher
Dec 24, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Md A NahidEdward K L Chan
May 18, 2011·Proceedings of the National Academy of Sciences of the United States of America·Alberto DávalosCarlos Fernández-Hernando
May 25, 2012·The British Journal of Nutrition·J A TurA Pons
Jul 24, 2012·The Journal of Clinical Investigation·Shu-Hao HsuKalpana Ghoshal
Jul 24, 2012·The Journal of Clinical Investigation·Wei-Chih TsaiAnn-Ping Tsou

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