MiR-146a modulates macrophage polarization by inhibiting Notch1 pathway in RAW264.7 macrophages

International Immunopharmacology
Cheng HuangJun Li

Abstract

Macrophages are heterogeneous and plastic cells which are able to undergo dynamic transition between M1 and M2 polarized phenotypes in response to the microenvironment signals. However, the underlying molecular mechanisms of macrophage polarization are still obscure. In the current study, it was revealed that miR-146a might play a pivotal role in macrophage polarization. As our results indicated, miR-146a was highly expressed in M2 macrophages rather than M1 macrophages. Over-expression of miR-146a resulted in significantly decreased production of pro-inflammatory cytokines including iNOS and TNF-α in M1 macrophages, while increased production of M2 marker genes such as Arg1 and CD206 in M2 macrophages. In contrast, knockdown of miR-146a promoted M1 macrophage polarization but diminished M2 macrophage polarization. Mechanistically, it was revealed that miR-146a modulated macrophage polarization by targeting Notch1. Of note, PPARγ was responsible as another target for miR-146a-mediated macrophage polarization. Taken together, it was suggested that miR-146a might serve as a molecular regulator in macrophage polarization and is a potential therapeutic target for inflammatory diseases.

References

Aug 4, 2006·Proceedings of the National Academy of Sciences of the United States of America·Konstantin D TaganovDavid Baltimore
Jan 24, 2007·Proceedings of the National Academy of Sciences of the United States of America·Ryan M O'ConnellDavid Baltimore
Mar 8, 2008·Arteriosclerosis, Thrombosis, and Vascular Biology·Elena RigamontiBart Staels
Jul 23, 2008·Nature Immunology·David BaltimoreKonstantin D Taganov
Nov 22, 2008·Biochemical Society Transactions·Andrew E WilliamsMark A Lindsay
Nov 26, 2008·Nature Reviews. Immunology·David M Mosser, Justin P Edwards
Dec 25, 2008·Annual Review of Immunology·Fernando O MartinezSiamon Gordon
Apr 22, 2009·Cell·Raphael Kopan, Maria Xenia G Ilagan
Jun 16, 2009·Current Opinion in Pharmacology·Eleni Tsitsiou, Mark A Lindsay
Aug 27, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hasina Hamilton OuttzJan Kitajewski
Sep 22, 2010·Nature Immunology·Subhra K Biswas, Alberto Mantovani
Jan 12, 2011·Circulation·Kisha PiggottCornelia M Weyand
Feb 15, 2011·The Journal of Clinical Investigation·Anca SindrilaruKarin Scharffetter-Kochanek
Jul 7, 2011·Molecular and Cellular Biology·Jie MeiChun-Li Zhang
Sep 14, 2011·Immunology and Cell Biology·Mohamed El Gazzar, Charles E McCall
Oct 15, 2011·Nature Reviews. Immunology·Peter J Murray, Thomas A Wynn
Oct 26, 2011·Nature Reviews. Immunology·Toby Lawrence, Gioacchino Natoli
Jan 27, 2012·Trends in Immunology·Brian RuffellLisa M Coussens
Mar 2, 2012·The Journal of Clinical Investigation·Antonio Sica, Alberto Mantovani
May 3, 2012·The Journal of Biological Chemistry·Joel W GraffMary E Wilson
Jun 1, 2012·Proceedings of the National Academy of Sciences of the United States of America·Alicia ArranzChristos Tsatsanis
Mar 19, 2013·Trends in Immunology·Mario Leonardo SquadritoMikael J Pittet
Apr 27, 2013·Nature·Thomas A WynnJeffrey W Pollard
May 4, 2013·Arteriosclerosis, Thrombosis, and Vascular Biology·Chiara BolegoGiulia Chinetti-Gbaguidi
May 25, 2013·Trends in Endocrinology and Metabolism : TEM·Tamás RőszerMercedes Ricote
Aug 27, 2013·Cancer Letters·Ingrid Espinoza, Lucio Miele
Jan 22, 2014·Nature Reviews. Immunology·Tracy Hussell, Thomas J Bell
Mar 29, 2014·F1000prime Reports·Fernando O Martinez, Siamon Gordon

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Citations

Apr 14, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Xiaoxue MengYanbin Song
Apr 30, 2017·Molecular Reproduction and Development·Sarah A RobertsonJohn E Schjenken
Sep 9, 2018·Glia·Marta FumagalliClaudia Verderio
Sep 19, 2019·Annual Review of Pathology·Massimo LocatiAlberto Mantovani
Jun 4, 2016·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Amanda CroasdellRichard P Phipps
Jun 5, 2020·Human & Experimental Toxicology·Z-F JiangJ Shen
Apr 26, 2019·Laboratory Investigation; a Journal of Technical Methods and Pathology·Sushan LiJinyue Hu
Oct 2, 2019·Frontiers in Immunology·Xiaoling Niu, Grant S Schulert
May 14, 2020·International Journal of Molecular Sciences·Cole LiechtyJunwang Xu
Aug 23, 2020·International Journal of Molecular Sciences·Xingwang WuYun Zheng
May 7, 2019·Frontiers in Immunology·Graziella CurtaleMassimo Locati
Jun 6, 2020·International Journal of Molecular Sciences·Nicole C SmithRune Andreassen
Oct 1, 2020·International Journal of Molecular Sciences·Bilash ChatterjeeAmit Kumar Srivastava
Jun 13, 2018·Frontiers in Immunology·Heng LiGuo-Jun Zhao
Feb 8, 2019·Npj Aging and Mechanisms of Disease·Ilja L Kruglikov, Philipp E Scherer
Jun 12, 2020·Immunology and Cell Biology·Jianting ShiShanping Jiang
Feb 7, 2021·Tissue Engineering and Regenerative Medicine·June Seok HeoHyun Ok Kim
Oct 31, 2020·Frontiers in Immunology·Igal Ifergan, Stephen D Miller
Dec 23, 2020·Thrombosis and Haemostasis·Ana B ArroyoRocío González-Conejero
Oct 31, 2020·Frontiers in Endocrinology·Gabriella Simões HeynKelly Grace Magalhães
Oct 18, 2019·Journal of Controlled Release : Official Journal of the Controlled Release Society·Sharon Wei Ling LeeValeria Chiono
Nov 7, 2020·Biochemical and Biophysical Research Communications·Lingling LiLing Fang
Oct 27, 2017·Translational Research : the Journal of Laboratory and Clinical Medicine·Arjun SaradnaPeisong Gao
May 1, 2021·Biomedicines·Melanie A KimmMoritz Wildgruber

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