miR-148a-3p Mediates Notch Signaling to Promote the Differentiation and M1 Activation of Macrophages

Frontiers in Immunology
Fei HuangHong-Yan Qin

Abstract

The Notch pathway plays critical roles in the differentiation and polarized activation of macrophages; however, the downstream molecular mechanisms underlying Notch activity in macrophages remain elusive. Our previous study has identified a group of microRNAs that mediate Notch signaling to regulate macrophage activation and tumor-associated macrophages (TAMs). In this study, we demonstrated that miR-148a-3p functions as a novel downstream molecule of Notch signaling to promote the differentiation of monocytes into macrophages in the presence of granulocyte macrophage colony-stimulating factor (GM-CSF). Meanwhile, miR-148a-3p promoted M1 and inhibited M2 polarization of macrophages upon Notch activation. Macrophages overexpressing miR-148a-3p exhibited enhanced ability to engulf and kill bacteria, which was mediated by excessive production of reactive oxygen species (ROS). Further studies using reporter assay and Western blotting identified Pten as a direct target gene of miR-148a-3p in macrophages. Macrophages overexpressing miR-148a-3p increased their ROS production through the PTEN/AKT pathway, likely to defend against bacterial invasion. Moreover, miR-148a-3p also enhanced M1 macrophage polarization and pro-inflammatory res...Continue Reading

References

Jan 1, 1996·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·T Honjo
Jun 3, 2000·The EMBO Journal·T Schroeder, U Just
Apr 20, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Eva MonsalveMaría José M Díaz-Guerra
Aug 21, 2007·Glia·Luc GrandbarbePaul Heuschling
Dec 19, 2007·European Journal of Immunology·Tanapat PalagaBarbara A Osborne
Sep 5, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Marie BenoitJean-Louis Mege
Nov 26, 2008·Nature Reviews. Immunology·David M Mosser, Justin P Edwards
Apr 22, 2009·Cell·Raphael Kopan, Maria Xenia G Ilagan
Apr 30, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Weijuan ZhangSidong Xiong
Aug 27, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hasina Hamilton OuttzJan Kitajewski
Sep 22, 2010·Nature Immunology·Subhra K Biswas, Alberto Mantovani
Jan 10, 2012·Annual Review of Immunology·Ryan M O'ConnellDavid Baltimore
Dec 12, 2012·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Peng ChengXiang-Hang Luo
Mar 19, 2013·Trends in Immunology·Mario Leonardo SquadritoMikael J Pittet
Apr 27, 2013·Nature·Thomas A WynnJeffrey W Pollard
May 15, 2013·Nature Reviews. Immunology·Freddy RadtkeFabienne Tacchini-Cottier
Jun 25, 2013·Free Radical Biology & Medicine·Shotaro Nakajima, Masanori Kitamura
May 9, 2014·Science·Ruth A FranklinMing O Li
May 24, 2014·Nature Reviews. Immunology·Florent Ginhoux, Steffen Jung
Jul 17, 2014·Nature Reviews. Molecular Cell Biology·Minju Ha, V Narry Kim
Aug 26, 2014·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Fei HeHua Han
Oct 9, 2014·Frontiers in Oncology·Amancio Carnero, Jesus M Paramio
Nov 5, 2014·Frontiers in Immunology·Paola Italiani, Diana Boraschi
Apr 12, 2015·Annual Review of Immunology·Chen VarolSteffen Jung
Apr 24, 2015·Molecular Therapy : the Journal of the American Society of Gene Therapy·Hon-Kit Andus WongAnna M Krichevsky
Oct 28, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Li ZhangLin Liu
Jan 23, 2016·American Journal of Physiology. Cell Physiology·Liu QingjuanLiu Shuxia
Feb 11, 2016·Biochemical Society Transactions·Nick R LeslieHelen M Wise
Feb 24, 2016·Nature Immunology·Alicia Gonzalez-MartinChangchun Xiao
Mar 22, 2016·Oxidative Medicine and Cellular Longevity·Jixiang ZhangWeiguo Dong
Aug 31, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Almudena Ruiz-GarcíaMaría José M Díaz-Guerra

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Citations

Jul 16, 2019·Expert Opinion on Therapeutic Targets·Giorgio AquilaAnna Malaschicheva
Jul 9, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ni ZhangHongyan Qin
Jun 20, 2018·Frontiers in Immunology·Fokhrul HossainLucio Miele
Jan 4, 2019·Journal of Translational Medicine·Bodo C Melnik, Gerd Schmitz
Apr 25, 2018·Frontiers in Immunology·Tanapat PalagaPatipark Kueanjinda
Oct 15, 2019·Frontiers in Immunology·Isabelle Duroux-RichardFlorence Apparailly
Jan 4, 2020·Journal of Zhejiang University. Science. B·Chong ChenYun-Ping Luo
Aug 1, 2018·Frontiers in Immunology·Mahnaz JanghorbanXiang H-F Zhang
May 21, 2019·Arthritis & Rheumatology·Dallas M RohraffAmr H Sawalha
Jun 14, 2019·Frontiers in Immunology·Francesco Vieceli Dalla SegaPaola Rizzo
Sep 20, 2020·Journal for Immunotherapy of Cancer·Liang WangHong-Yan Qin
Jan 10, 2020·Oxidative Medicine and Cellular Longevity·Giorgio AquilaPaola Rizzo
Jan 1, 2021·Frontiers in Oncology·Jane KooAlisa B Lee-Sherick
Nov 19, 2020·Clinical & Translational Immunology·Andrew D FoersKen C Pang
May 25, 2021·Frontiers in Molecular Biosciences·Ping LinShou-Dong Guo
Jul 13, 2021·Frontiers in Immunology·Pavithra SampathRamalingam Bethunaickan
Aug 28, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Soudeh Ghafouri-FardMohammad Samadian

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Datasets Mentioned

BETA
GM-CSF

Methods Mentioned

BETA
PCR
Transfection
Assay
Protein Assay
FACS
X-ray
ELISA
nuclear translocation

Software Mentioned

miRDB
PicTar
Graph Pad Prism
Image Pro Plus
Target Scan

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