miR-148a-3p overexpression contributes to glomerular cell proliferation by targeting PTEN in lupus nephritis

American Journal of Physiology. Cell Physiology
Liu QingjuanLiu Shuxia

Abstract

The objective of this study was to investigate the role of miR-148a-3p in lupus nephritis (LN) based on data from previous studies and a microRNA assay. We evaluated the miR-148a-3p expression level in LN renal tissues and blood serum to determine its clinicopathological significance and effect on glomerular cell proliferation. Then, we collected renal glomeruli from LN mice and determined the miR-148a-3p, proliferating cell nuclear antigen (PCNA), and PCNA/Thy1 expression. We performed functional analyses of miR-148a-3p in vitro and in vivo. We also investigated the target gene of miR-148a-3p in LN. The results showed that miR-148a-3p expression levels were significantly higher not only in glomeruli but also in the blood serum during LN and increased in the glomeruli of LN mice and that at the same time there was positive correlation between miR-148a-3p and PCNA expression of glomruli. Overexpression of miR-148a-3p accelerated cell proliferation and PCNA expression, while a miR-148a-3p inhibitor inhibited cell proliferation via the Akt/cyclin D1 pathway. Furthermore, miR-148a-3p overexpression reduced the phosphatase and tensin homology deleted on chromosome ten (PTEN) expression level, while miR-148a-3p silencing increased it...Continue Reading

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Citations

Sep 18, 2016·Autoimmunity Reviews·Ji-Qing ChenMargit Zeher
Jun 2, 2017·International Journal of Cancer. Journal International Du Cancer·Abeer Al-QatatiHeidi Schwarzenbach
Jun 2, 2017·Current Opinion in Rheumatology·Xiong LeNan Shen
Feb 11, 2018·In Vitro Cellular & Developmental Biology. Animal·Hongbing HeSongjia Lai
Feb 23, 2019·Journal of Cellular Physiology·Chengchuang SongHong Chen
Feb 25, 2017·Journal of the American Society of Nephrology : JASN·Piera Trionfini, Ariela Benigni
May 1, 2019·International Braz J Urol : Official Journal of the Brazilian Society of Urology·Caixiang ZhangYe Tian
Jun 6, 2018·Frontiers in Immunology·Takayuki KatsuyamaVaishali R Moulton
Apr 10, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Nina BoxbergerUwe K Zettl
Apr 4, 2021·Animals : an Open Access Journal From MDPI·Xiayang JinYuzhu Luo

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