miR-149-5p inhibits cell growth by regulating TWEAK/Fn14/PI3K/AKT pathway and predicts favorable survival in human osteosarcoma

International Journal of Immunopathology and Pharmacology
Ruida XuLi-Feng Lao

Abstract

MicroRNAs (miRNAs) as small non-coding RNAs act as either tumor suppressors or oncogenes in human cancers, of which miR-149-5p (miR-149) is involved in tumor growth and metastasis, but its role and molecular mechanisms underlying osteosarcoma growth are poorly understood. The correlation of miR-149 expression with clinicopathological characteristics and prognosis in patients with sarcoma was analyzed by The Cancer Genome Atlas (TCGA) RNA-sequencing data. Osteosarcoma cell growth affected by miR-149 was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and colony formation assays. As a result, we found that the expression level of miR-149 was markedly downregulated in human sarcoma samples and were negatively associated with tumor size, acting as an independent prognostic factor for overall survival of the sarcoma patients. Restoration of miR-149 expression suppressed osteosarcoma cell growth, while its knockdown reversed these effects. Furthermore, we identified TNFRSF12A (TNF receptor superfamily member 12A), also called fibroblast growth factor-inducible 14 (Fn14) as a direct target of miR-149, and TNFRSF12A and its ligand TNFSF12 (TNF superfamily member 12), also called tumor necrosis factor-rel...Continue Reading

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Citations

Mar 21, 2020·Signal Transduction and Targeted Therapy·Jawad FaresYoussef Fares
Apr 17, 2020·Signal Transduction and Targeted Therapy·Jawad FaresYoussef Fares
Apr 2, 2021·Cancer Management and Research·Jinhua MaXi Qu
Nov 17, 2020·Pathology, Research and Practice·Ning WangJiazeng Xia
May 1, 2021·Animals : an Open Access Journal From MDPI·Hongfang GuoLinsen Zan
Jun 3, 2021·International Journal of Molecular Sciences·Clément VeysPhilippe Galéra
Jun 15, 2021·Frontiers in Endocrinology·Jiajie TuZhiying Yu

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Methods Mentioned

BETA
electrophoresis
Assay
transfection

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