miR-150 enhances apoptotic and anti-tumor effects of paclitaxel in paclitaxel-resistant ovarian cancer cells by targeting Notch3

Oncotarget
Tae Hoen KimHee-Jung An

Abstract

Tumor recurrence by obtaining chemoresistance is a major obstacle to treating ovarian cancer. By TargetScan database and a luciferase reporter assay, we identified miR-150 directly targets Notch3, which is a key oncogene in ovarian cancer. We, therefore, investigated the role of miR-150 in ovarian cancer cells, and the usefulness of miR-150 as a therapeutic target in chemoresistant ovarian cancer, through examining miR-150 expression by qRT-PCR in ovarian cancer cell lines and tissues, and assessing the gain-of-function effect by WST, colony forming, TUNEL, wound healing and angiogenesis assays. Western blotting was performed to evaluate its downstream targets. The miR-150 expression was significantly downregulated in ovarian cancers. Treatment with pre-miR-150 significantly inhibited cancer cell proliferation, and induced apoptosis in PTX (paclitaxel) -resistant SKpac cells, which was not seen by PTX only treatment. On spheroid forming assay, an additional pre-miR-150 treatment with PTX decreased cancer stem cell activation in PTX-resistant SKpac cells. An experimental upregulation of miR-150 also decreased cancer cell migration and angiogenesis in SKpac cells. The Notch3 downstream proteins(NICD3 and HEY2), and cell cycle-rel...Continue Reading

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Citations

Jul 10, 2019·Current Drug Targets·Seyed Mostafa ParizadehAmir Avan
Apr 7, 2018·The Oncologist·Zviadi AburjaniaJ Bart Rose
Mar 27, 2020·Biomolecules·Samira Hosseini-Alghaderi, Martin Baron
Jul 16, 2019·Cell & Bioscience·Ying ZhangLiu Yang
Sep 6, 2019·Medicina·Ionut Andrei PaunescuCatalin Marian
Jan 1, 2020·Cancers·Mohammad Hasan SoheilifarZhixiang Wang
Aug 19, 2020·Journal of Gynecologic Oncology·Yanwei Luo, Rong Gui
Jun 19, 2020·Cancer Cell International·Yanbin LiuYali Huang
Dec 29, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Soudeh Ghafouri-FardMohammad Taheri
Feb 15, 2021·Gynecologic Oncology·Maryam HashemipourHamed Mirzaei
Apr 23, 2020·Cell Death & Disease·Mariusz L Hartman, Malgorzata Czyz
Apr 4, 2021·Cancers·Shailendra Kumar Dhar DwivediResham Bhattacharya
Apr 9, 2021·Life Sciences·Pawan Kumar Raghav, Zoya Mann
Aug 24, 2020·Pathology, Research and Practice·Maryam AkbarzadehMaryam Majidinia
Nov 13, 2021·International Journal of Radiation Oncology, Biology, Physics·Marshleen YadavNaduparambil K Jacob

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Methods Mentioned

BETA
transfection
PCR
fluorescence activated cell sorting
scraping

Software Mentioned

Image [UNK]
TargetScan
[UNK] XRS +
SPSS

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