miR-150 regulates obesity-associated insulin resistance by controlling B cell functions

Scientific Reports
Wei YingBeiyan Zhou

Abstract

Adipose tissue resident B cells account for more than 20% of stromal cells within visceral adipose tissues; however, their functions in the adipose tissue niche are poorly elucidated. Here we report that miR-150 modulates adipose tissue function by controlling activation of B cells and their interactions with other immune cells. miR-150KO mice displayed exacerbated obesity-associated tissue inflammation and systemic insulin resistance, which is recapitulated by adoptive transfer of B cells, but not purified immunoglobulin, into obese B(null) mice. Using purified cell populations, we found that enhanced proinflammatory activation of adipose tissue T cells and macrophages was due to miR-150KO B cells action but not cell-autologous mechanisms. miR-150KO B cells displayed significantly enhanced antigen presentation upon stimulation, ultimately leading to elevated inflammation and insulin resistance, compared to wild type B cells. Knockdown of identified miR-150 target genes, Elk1, Etf1 or Myb attenuated B cell action by altering B cell receptor pathways and MHCII cell surface presentation. Our results demonstrate a critical role for miR-150 in regulating B cell functions in adipose tissue which ultimately regulate both metabolic an...Continue Reading

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Citations

Jan 6, 2017·Epigenetics : Official Journal of the DNA Methylation Society·Esma KarkeniJean-François Landrier
Feb 14, 2017·The Journal of Clinical Investigation·Wei YingJerrold M Olefsky
Jan 24, 2018·Metabolic Syndrome and Related Disorders·Elizabeth MaW Timothy Garvey
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Jul 25, 2021·International Journal of Molecular Sciences·Vianet Argelia Tello-FloresEugenia Flores-Alfaro
Aug 19, 2021·Journal of Molecular Cell Biology·Cheng Hu, Weiping Jia

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Methods Mentioned

BETA
flow cytometry
PCR
electrophoresis
ELISA
Assay
transfection

Software Mentioned

Kaluza
TargetScan Mouse
Intelligent Innovations
Plex Data Pro TM
PicTar
Bio
Accuri
Flowjo
Graphpad Prism

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