miR-155, identified as anti-metastatic by global miRNA profiling of a metastasis model, inhibits cancer cell extravasation and colonization in vivo and causes significant signaling alterations

Oncotarget
Karina G ThomsenHenrik J Ditzel

Abstract

To gain insight into miRNA regulation in metastasis formation, we used a metastasis cell line model that allows investigation of extravasation and colonization of circulating cancer cells to lungs in mice. Using global miRNA profiling, 28 miRNAs were found to exhibit significantly altered expression between isogenic metastasizing and non-metastasizing cancer cells, with miR-155 being the most differentially expressed. Highly metastatic mesenchymal-like CL16 cancer cells showed very low miR-155 expression, and miR-155 overexpression in these cells lead to significantly decreased tumor burden in lungs when injected intravenously in immunodeficient mice. Our experiments addressing the underlying mechanism of the altered tumor burden revealed that miR-155-overexpressing CL16 cells were less invasive than CL16 control cells in vitro, while miR-155 overexpression had no effect on cancer cell proliferation or apoptosis in established lung tumors. To identify proteins regulated by miR-155 and thus delineate its function in our cell model, we compared the proteome of xenograft tumors derived from miR-155-overexpressing CL16 cells and CL16 control cells using mass spectrometry-based proteomics. >4,000 proteins were identified, of which 9...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Aug 3, 2002·Nature Reviews. Cancer·Ann F ChambersIan C MacDonald
Nov 5, 2003·Methods : a Companion to Methods in Enzymology·Gordon K Smyth, Terry Speed
Oct 6, 2004·Genome Biology·Robert C GentlemanJianhua Zhang
Mar 2, 2005·Proceedings of the National Academy of Sciences of the United States of America·Peggy S EisJames E Dahlberg
May 26, 2005·Rapid Communications in Mass Spectrometry : RCM·Anne K CallesenOle N Jensen
Jul 19, 2005·Cancer Research·Steve GoodisonVirginia Urquidi
Aug 2, 2005·Nature Reviews. Cancer·Britta WeigeltLaura J van 't Veer
Aug 17, 2005·Cancer Research·Marilena V IorioCarlo M Croce
Feb 8, 2006·Proceedings of the National Academy of Sciences of the United States of America·Stefano VoliniaCarlo M Croce
Mar 15, 2006·Cancer Cell·Nozomu YanaiharaCurtis C Harris
May 23, 2006·BMC Bioinformatics·Matthew E RitchieGordon K Smyth
Aug 1, 2006·The American Journal of Pathology·Mika SuzukiDavid Tarin
Aug 22, 2006·Journal of Pharmacological Sciences·Soken TsuchiyaGozoh Tsujimoto
Apr 21, 2009·Nature Methods·Jacek R WiśniewskiMatthias Mann
Jun 25, 2009·International Journal of Cancer. Journal International Du Cancer·Thomas GreitherHelge Taubert
Aug 25, 2009·Differentiation; Research in Biological Diversity·Valerie MontelDavid Tarin
Dec 24, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Emmanuelle Charafe-JauffretMax S Wicha
Feb 19, 2010·Journal of the National Cancer Institute·Zeshaan A RasheedWilliam Matsui
Apr 8, 2010·The Journal of Biological Chemistry·William KongJin Q Cheng
Aug 17, 2010·Nature Chemical Biology·Isao MiyazakiHiroyuki Osada
Nov 4, 2010·Nature Reviews. Clinical Oncology·Nicole M A WhiteGeorge M Yousef
Apr 26, 2011·The American Journal of Pathology·Silvia LicciulliMyriam Alcalay
Nov 23, 2011·Journal of Surgical Oncology·Jian ChenJin-Hai Tang

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Citations

Dec 25, 2019·International Archives of Occupational and Environmental Health·Imene AmraniJean-Marc Lo-Guidice
Aug 28, 2020·Human Fertility : Journal of the British Fertility Society·Loubna DrissennekDelphine Haouzi
May 6, 2020·The Journal of International Medical Research·Yingyuan LiZhongxing Wang
Dec 17, 2020·Cancers·Tomasz M GrzywaPaweł K Włodarski
Feb 10, 2021·Biology·Francisco Perez-DominguezFrancisco Aguayo
Aug 7, 2019·Seminars in Cancer Biology·Francesca OrsoDaniela Taverna

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Methods Mentioned

BETA
xenograft
bioluminescence imaging
Reverse Transcription PCR
PCR

Software Mentioned

LOESS
Proteome Discoverer
miRCURY
LIMMA ( Linear Models for Microarray Data )
Percolator
Caliper Life Science Living image
Normexp
Qlucore Omics Explorer
R
SEQUEST

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