MiR-155 inhibits cell migration of human cardiomyocyte progenitor cells (hCMPCs) via targeting of MMP-16.

Journal of Cellular and Molecular Medicine
Jia LiuJoost P G Sluijter

Abstract

Undesired cell migration after targeted cell transplantation potentially limits beneficial effects for cardiac regeneration. MicroRNAs are known to be involved in several cellular processes, including cell migration. Here, we attempt to reduce human cardiomyocyte progenitor cell (hCMPC) migration via increasing microRNA-155 (miR-155) levels, and investigate the underlying mechanism. Human cardiomyocyte progenitor cells (hCMPCs) were transfected with pre-miR-155, anti-miR-155 or control-miR (ctrl-miR), followed by scratch- and transwell-assays. These functional assays displayed that miR-155 over-expression efficiently inhibited cell migration by 38 ± 3.6% and 59 ± 3.7% respectively. Conditioned medium from miR-155 transfected cells was collected and zymography analysis showed a significant decrease in MMP-2 and MMP-9 activities. The predicted 3'-UTR of MMP-16, an activator of MMP-2 and -9, was cloned into the pMIR-REPORT vector and luciferase assays were performed. Introduction of miR-155 significantly reduced luciferase activity which could be abolished by cotransfection with anti-miR-155 or target site mutagenesis. By using MMP-16 siRNA to reduce MMP-16 levels or by using an MMP-16 blocking antibody, hCMPC migration could be b...Continue Reading

References

Nov 16, 2004·Journal of Vascular Research·Joost P G SluijterDominique P V de Kleijn
Nov 26, 2008·Investigative Ophthalmology & Visual Science·Dongsheng YanJia Qu
Mar 7, 2009·Brain Research·Hongping XiaMarie C Lin
Apr 23, 2009·Journal of Cellular and Molecular Medicine·L M PopescuMaria Simonetta Faussone-Pellegrini
Sep 3, 2009·The Journal of Biological Chemistry·Guisheng SongLi Wang
Jan 16, 2010·The Journal of Biological Chemistry·Yanyan TianZhihua Liu
Jun 17, 2010·Journal of Cellular and Molecular Medicine·Jia LiuJoost P G Sluijter
Jun 26, 2010·The International Journal of Cardiovascular Imaging·Tycho I G van der SpoelSteven A J Chamuleau
Jul 17, 2010·JACC. Cardiovascular Imaging·Mariann GyöngyösiGerald Maurer
Jul 27, 2010·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Chengwang XuXiaofei Zheng
Aug 10, 2010·American Journal of Physiology. Lung Cellular and Molecular Physiology·Joy SarkarJ Usha Raj
Aug 17, 2010·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Hui-Ju WangHou-Quan Tao
Feb 11, 2011·Journal of Cellular and Molecular Medicine·Angelique A M van OorschotMarie-José Goumans

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Citations

Jul 31, 2013·Journal of Cardiovascular Translational Research·J C DeddensJ P G Sluijter
Aug 4, 2012·Circulation Research·Xinghui Sun, Mark W Feinberg
Jul 6, 2014·Neuropathology and Applied Neurobiology·Shahrzad JalaliGelareh Zadeh
Jan 28, 2014·Biochimica Et Biophysica Acta·Sherif Abdelaziz IbrahimMartin Götte
Apr 4, 2013·Critical Reviews in Biochemistry and Molecular Biology·Jennifer VandoorenGhislain Opdenakker
May 28, 2016·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Fan MengMichael W Crowder
May 18, 2016·Journal of Cellular Physiology·Rahul MittalXue Zhong Liu
Jan 1, 2013·Genes·Duane JeansonneFrancesca Peruzzi
Nov 22, 2017·PloS One·Paula Paccielli FreireRobson Francisco Carvalho
Jan 29, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Meiwan CaoJunko Kimura
Feb 23, 2020·American Journal of Physiology. Heart and Circulatory Physiology·Amanda R KaminskiMerry L Lindsey
Apr 3, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Fangfang LiYumin Luo
Dec 21, 2018·International Journal of Molecular Medicine·Zhen-Dong ZhuXuan Zhang
Jun 14, 2019·Oxidative Medicine and Cellular Longevity·Jing GuoZhi-Min Du

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Methods Mentioned

BETA
Transfection
PCR
zymography

Software Mentioned

Image J
Photoshop

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