miR-155 is dispensable in monosodium urate-induced gouty inflammation in mice

Arthritis Research & Therapy
Qibin YangJingguo Zhou

Abstract

The findings of a previous study by Jin et al. have shown that microRNA (miR)-155 was upregulated in patients with acute gouty arthritis and enhanced the proinflammatory cytokines. There is no direct evidence to support that miR-155 is indeed involved in monosodium urate (MSU)-induced inflammatory responses in vivo. The aim of this study was to investigate the role of miR-155 knock-out (KO) or knock-in (KI) mice in MSU-induced animal models to mimic acute gout. MiR-155 expression in cultured bone marrow-derived macrophages (BMDMs) from miR-155 KO, miR-155 KI, and wild-type (WT) mice treated with MSU crystals in vitro was detected by real-time quantitative polymerase chain reaction (qPCR). MiR-155 KO and WT mice were used to induce an acute gouty inflammatory response with MSU crystals including models of foot pad inflammation, ankle arthritis, air pouch inflammation, and peritonitis. Furthermore, the proinflammatory interleukin (IL)-1β levels in lavage fluids from air pouch and peritoneal cavity models were measured by enzyme-linked immunosorbent assay (ELISA), and tumor necrosis factor (TNF)-α production from BMDMs of miR-155 KI mice treated with MSU were measured by flow cytometry. MiR-155 expression was quickly upregulated i...Continue Reading

References

Aug 1, 1995·Arthritis and Rheumatism·B N CronsteinG Firestein
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Apr 3, 2008·Arthritis and Rheumatism·Joanna StanczykDiego Kyburz
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Dec 3, 2014·Clinical Rheumatology·Haiyan ChenXiaoxia Zhu

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Citations

Aug 16, 2018·Arthritis Research & Therapy·Qibin YangJingguo Zhou
Oct 2, 2019·Frontiers in Immunology·Xiaoling Niu, Grant S Schulert
Nov 18, 2020·International Journal of Rheumatic Diseases·Guangwen LiGe Wang
Jul 22, 2021·BMC Complementary Medicine and Therapies·Hao-Fei FanQi-Bing Liu
Jul 12, 2021·Arthritis Research & Therapy·Jana BohatáBlanka Stibůrková

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Methods Mentioned

BETA
lavage
flow cytometry
Assay
ELISA

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