miR-15a and miR-16 regulate serotonin transporter expression in human placental and rat brain raphe cells

The International Journal of Neuropsychopharmacology
Pablo R MoyaDennis L Murphy

Abstract

The serotonin transporter (SERT) is a key regulatory molecule in serotonergic transmission implicated in numerous biological processes relevant to human disorders. Recently, it was shown that SERT expression is controlled by miR-16 in mouse brain. Here, we show that SERT expression is regulated additionally by miR-15a as well as miR-16 in human and rat tissues. This post-transcriptional regulation was observed and characterized in reporter assays and likewise when endogenous SERT expression was evaluated in human placental choriocarcinoma JAR cells and rat brain raphe RN46A cells - two cell lines that endogenously express SERT. Similar effects for miR-16 to those of miR-15a were found in both human and rat cell lines. The effects of miR-15a and miR-16 were comparable in extent to those originally reported for miR-16 in mice. These findings represent a novel layer of complexity for SERT expression regulation exerted by the mir-15a/16 cluster, whose genes are adjacently located at human chromosome 13q14.3.

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Citations

Jan 22, 2013·ACS Chemical Neuroscience·Dennis L MurphyNicholas M Vogt
Jun 27, 2013·Proceedings of the National Academy of Sciences of the United States of America·YoneJung YoonCharles E Glatt
Apr 1, 2014·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Pia B DanborgNiels H H Heegaard
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Jan 24, 2021·Neurochemical Research·Esperanza JiménezFrancisco Zafra
Apr 22, 2019·Current Problems in Cardiology·Yeshun WuChun Yang

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