MiR-15b and miR-322 inhibit SETD3 expression to repress muscle cell differentiation

Cell Death & Disease
Meng-Jie ZhaoHai-Ning Du

Abstract

SETD3 is a member of SET-domain containing methyltransferase family, which plays critical roles in various biological events. It has been shown that SETD3 could regulate the transcription of myogenic regulatory genes in C2C12 differentiation and promote myoblast determination. However, how SETD3 is regulated during myoblast differentiation is still unknown. Here, we report that two important microRNAs (miRNAs) could repress SETD3 and negatively contribute to myoblast differentiation. Using microRNA (miRNA) prediction engines, we identify and characterize miR-15b and miR-322 as the primary miRNAs that repress the expression of SETD3 through directly targeting the 3'-untranslated region of SETD3 gene. Functionally, overexpression of miR-15b or miR-322 leads to the repression of endogenous SETD3 expression and the inhibition of myoblast differentiation, whereas inhibition of miR-15b or miR-322 derepresses endogenous SETD3 expression and facilitates myoblast differentiation. In addition, knockdown SETD3 in miR-15b or miR-322 repressed myoblasts is able to rescue the facilitated differentiation phenotype. More interestingly, we revealed that transcription factor E2F1 or FAM3B positively or negatively regulates miR-15b or miR-322 exp...Continue Reading

References

Nov 16, 2002·Proceedings of the National Academy of Sciences of the United States of America·George Adrian CalinCarlo M Croce
Dec 11, 2002·Current Opinion in Cell Biology·Timothy A McKinseyEric N Olson
Aug 16, 2005·Seminars in Cell & Developmental Biology·Charlotte A Berkes, Stephen J Tapscott
Aug 23, 2006·The Journal of Cell Biology·Hak Kyun KimAnindya Dutta
Jan 13, 2009·Biochemical and Biophysical Research Communications·Hongping XiaMarie C Lin
Feb 12, 2009·BMC Genomics·Tara G McDaneldRalph T Wiedmann
Mar 31, 2009·Cold Spring Harbor Symposia on Quantitative Biology·M A RudnickiS Kuang
Jun 6, 2009·Cell Death and Differentiation·R I AqeilanC M Croce
Apr 21, 2010·Molecular and Cellular Biology·María J BuenoMarcos Malumbres
Jul 8, 2010·Immunity·Stefan KuchenRafael Casellas
Jun 30, 2011·The British Journal of Dermatology·A IchiharaH Ihn
Aug 13, 2011·The Journal of Biological Chemistry·Gwang Hyeon EomSang-Beom Seo
Mar 26, 2013·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Wen LuoXiquan Zhang
Apr 9, 2013·The Journal of Biological Chemistry·Beatriz GámezFrancesc Ventura
Jun 13, 2013·Current Opinion in Genetics & Development·Abraham P Fong, Stephen J Tapscott
Jan 5, 2014·The Journal of Biological Chemistry·Antonis AntoniouLeonidas A Phylactou
Feb 15, 2014·Developmental Cell·Margaret Buckingham, Peter W J Rigby
May 28, 2014·Acta Biochimica Et Biophysica Sinica·Peiyue DongShiduo Sun
May 27, 2015·International Journal of Biological Macromolecules·S Vimalraj, N Selvamurugan
Sep 2, 2015·Proceedings of the National Academy of Sciences of the United States of America·Francesca LovatCarlo M Croce
Aug 12, 2016·Proceedings of the National Academy of Sciences of the United States of America·Xiaopeng ShenYu Liu
Aug 25, 2017·American Journal of Physiology. Cell Physiology·Meiqiang ChuLingjiang Min
Nov 21, 2017·Seminars in Cell & Developmental Biology·Michael V Taylor, Simon M Hughes
Dec 12, 2018·ELife·Sebastian KwiatkowskiJakub Drozak

❮ Previous
Next ❯

Citations

Feb 20, 2020·Current Opinion in Clinical Nutrition and Metabolic Care·Anthony J SannicandroKatarzyna Goljanek-Whysall
Jun 13, 2020·International Journal of Molecular Sciences·Veronika S GeorgievaBent Brachvogel
Jan 30, 2020·Scientific Reports·Takahiro Nemoto, Yoshihiko Kakinuma
Nov 7, 2020·Biochimica Et Biophysica Acta. Reviews on Cancer·Wen-Jie Shu, Hai-Ning Du
Dec 29, 2020·Frontiers in Cell and Developmental Biology·Chen HeChangjun Li
Dec 12, 2021·Journal of Muscle Research and Cell Motility·Zsuzsanna GaálLászló Csernoch
Jan 13, 2022·Cellular and Molecular Life Sciences : CMLS·Tingting ZouZhenghong Lin

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
transfection

Software Mentioned

TargetScan
ImageJ
miRanda
Prism

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.