MiR-16-5p inhibits breast cancer by reducing AKT3 to restrain NF-κB pathway

Bioscience Reports
Liwei Ruan, Xiaojun Qian

Abstract

Background: Breast cancer endangers the life of women and has become the major cause of deaths among them. MiRNAs are found to exert a regulatory effect on the migration, proliferation and apoptosis of breast cancer cells. This research aims at investigating the miR-16-5p expression and its effect on the pathogenesis of breast cancer. Methods: Their clinical data were analyzed with qRT-PCR. CCK8, EdU and Transwell was performed to explore the function of miR-16-5p in cell migration and proliferation of breast cancer cells. Dual-luciferase reporter assay, immunohistochemistry and Western blotting were carried out to explore the relation between miR-16-5p and AKT3. Results: It was discovered that miR-16-5p was lowly expressed in breast cancer patients. Meanwhile, breast cancer patients with under-expressed miR-16-5p had a lower survival rate than those with highly expressed miR-16-5p. Furthermore, decreased miR-16-5p in cell and animal models enhanced migration and proliferation of breast cancer cells, stimulated cell cycle and reduced cell apoptosis. Finally, we found miR-16-5p restrained the NF-κB pathway and decreased AKT3 gene, thereby suppressing the breast cancer development. Conclusion: It can be seen that miR-16-5p exhibi...Continue Reading

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Oct 2, 2020·Neurochemical Research·Xiao ZhangHaiyan Yang
Jul 17, 2020·Non-coding RNA·Jun Sheng Wong, Yoke Kqueen Cheah
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Jul 30, 2021·Environmental Toxicology·Hui XiongJin Zhou

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Methods Mentioned

BETA
transfection
transfect
flow cytometry
flow
PCR

Software Mentioned

SPSS20
TargetScan
GraphPad
REGRNA

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