miR-181a/b control the assembly of visual circuitry by regulating retinal axon specification and growth

Developmental Neurobiology
Sabrina CarrellaIvan Conte

Abstract

Connectivity and function of neuronal circuitry require the correct specification and growth of axons and dendrites. Here, we identify the microRNAs miR-181a and miR-181b as key regulators of retinal axon specification and growth. Loss of miR-181a/b in medaka fish (Oryzias latipes) failed to consolidate amacrine cell processes into axons and delayed the growth of retinal ganglion cell (RGC) axons. These alterations were accompanied by defects in visual connectivity and function. We demonstrated that miR-181a/b exert these actions through negative modulation of MAPK/ERK signaling that in turn leads to RhoA reduction and proper neuritogenesis in both amacrine cells and RGCs via local cytoskeletal rearrangement. Our results identify a new pathway for axon specification and growth unraveling a crucial role of miR-181a/b in the proper establishment of visual system connectivity and function.

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Citations

Apr 14, 2019·EMBO Molecular Medicine·Alessia IndrieriBrunella Franco
Jun 11, 2020·Clinical & Experimental Ophthalmology·Lan-Fang SunZi-Bing Jin
Apr 27, 2018·Cellular and Molecular Life Sciences : CMLS·Sophie M HapakSourav Ghosh
Sep 7, 2018·Human Genetics·Marianthi Karali, Sandro Banfi
Mar 22, 2020·International Journal of Molecular Sciences·Alessia IndrieriBrunella Franco
Feb 9, 2021·Frontiers in Cell and Developmental Biology·Julia Sophie PawlickVolker Busskamp

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Methods Mentioned

BETA
transgenic
gene knockdown
confocal microscopy
protein assay
mechanical dissociation

Software Mentioned

Lab
NI Vision Development
Visionworks
ImageJ
‐ View

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