MiR-181b suppress glioblastoma multiforme growth through inhibition of SP1-mediated glucose metabolism

Cancer Cell International
JianXing YinXieFeng Wang

Abstract

Glucose metabolic reprogramming is a significant hallmark of malignant tumors including GBM. Previous studies suggest that microRNAs play key roles in modulating this process in GBM cells. miR-181b acts as a tumor suppressor miRNA in influencing glioma tumorigenesis. Our previous results showed that miR-181b was down-regulated in glioma cells and tissues. The extracellular acidification rate (ECAR), colony formation assay and levels of Glut1 and PKM2 were measured to assess the glucose metabolic and proliferation changes in GBM cells overexpressing miR-181b. Immunoblotting and luciferase reporter assay were performed to confirm the expression and role of SP1 as a direct target of miR-181b. ChIP assay was used to figure out the transcriptional regulation of SP1 on Glut1 and PKM2. In vivo study was examined for the role of miR-181b in GBM cells. MiR-181b overexpression significantly reduced the glucose metabolic and colony formation ability of GBM cells. And, SP1 was confirmed as a direct target of miR-181b while upregulation of SP1 could reverse the influence of overexpression of miR-181b. Furthermore, Glut1 and PKM2 could be regulated by SP1. Finally, miR-181b could inhibit the tumor growth in vivo. Our article demonstrated the...Continue Reading

References

Nov 26, 1999·Gene·G Suske
Jun 26, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·James C YaoKeping Xie
Sep 16, 2005·American Journal of Physiology. Cell Physiology·Daw-Yang Hwang, Faramarz Ismail-Beigi
Nov 30, 2005·Nucleic Acids Research·Caifu ChenKarl J Guegler
Jul 10, 2007·Acta Neuropathologica·David N LouisPaul Kleihues
Mar 28, 2008·Biochemical and Biophysical Research Communications·Inken Wierstra
May 23, 2009·Science·Matthew G Vander HeidenCraig B Thompson
Mar 8, 2011·Cell·Douglas Hanahan, Robert A Weinberg
Jun 8, 2011·Nature Reviews. Clinical Oncology·Maria Angelica CortezGeorge A Calin
Dec 31, 2011·Journal of Cancer Research and Clinical Oncology·Xie-Feng WangYong-Ping You
Mar 24, 2012·Cancer Cell·Douglas Hanahan, Lisa M Coussens
Sep 3, 2013·Nature Neuroscience·William A FlavahanAnita B Hjelmeland
Dec 5, 2013·Molecular Cancer·Shanmugasundaram Ganapathy-Kanniappan, Jean-Francois H Geschwind
Mar 12, 2015·The Journal of Craniofacial Surgery·Sheng NieSun Jie
Apr 30, 2016·Genes & Development·Talya L DaytonMatthew G Vander Heiden
Jun 18, 2016·Cancer Biomarkers : Section a of Disease Markers·Liang-Qing LiWen-Jun Xie
Nov 22, 2018·The Biochemical Journal·Geoffrey D Holman

❮ Previous
Next ❯

Citations

Jan 14, 2021·Clinical Oral Investigations·Agnes AssaoDenise Tostes Oliveira
Nov 28, 2020·Cancer Letters·Susi ZhuCong Peng
Jan 29, 2021·International Journal of Molecular Sciences·Dexter L PuckettAhmed Bettaieb
Jun 3, 2021·International Journal of Molecular Sciences·Anna Maria ReussNicolai Savaskan
Jun 5, 2021·Toxicology and Applied Pharmacology·Yunhui ZhangZhiying Huang

❮ Previous
Next ❯

Methods Mentioned

BETA
surgical resection
Assay
transfection
immunoprecipitation
PCR
xenograft
ChIP

Software Mentioned

microT
Targetscan
miRWalk
GEPIA

Related Concepts

Related Feeds

Cancer Metabolic Reprogramming

Cancer metabolic reprogramming is important for the rapid growth and proliferation of cancer cells. Cancer cells have the ability to change their metabolic demands depending on their environment, regulated by the activation of oncogenes or loss of tumor suppressor genes. Here is the latest research on cancer metabolic reprogramming.

Cancer Metabolic Reprogramming (Keystone)

Cancer metabolic reprogramming is important for the rapid growth and proliferation of cancer cells. Cancer cells have the ability to change their metabolic demands depending on their environment, regulated by the activation of oncogenes or loss of tumor suppressor genes. Here is the latest research on cancer metabolic reprogramming.

Cancer Metabolism

In order for cancer cells to maintain rapid, uncontrolled cell proliferation, they must acquire a source of energy. Cancer cells acquire metabolic energy from their surrounding environment and utilize the host cell nutrients to do so. Here is the latest research on cancer metabolism.