MiR-181c modulates the proliferation, migration, and invasion of neuroblastoma cells by targeting Smad7

Acta Biochimica Et Biophysica Sinica
Yong LiWu Yue

Abstract

MicroRNAs (miRNAs) function as key regulators of gene expression in various cancers. In this study, the aim is to explore the roles and regulation mechanism of miR-181c in neuroblastoma (NB) cells. We found that miR-181c was downregulated in metastatic NB tissues, compared with primary NB tissues. Then functional studies indicated that miR-181c overexpression inhibited NB cell proliferation, migration, and invasion, while miR-181c inhibition increased cell proliferation, migration, and invasion. EGFP reporter assay, real-time polymerase chain reaction and western blot analysis validated that Smad7 was a direct target of miR-181c. MiR-181c reduced Smad7 expression at both mRNA and protein levels. Finally, functional assays showed that the effect of Smad7 knockdown on cells was similar to that of miR-181c overexpression. Importantly, Smad7 overexpression could restore the antitumor effects that were induced by miR-181c. In conclusion, our results demonstrated that miR-181c inhibits NB cell growth and metastasis-related traits through the suppression of Smad7, functioning as a tumor suppressor. Moreover, our results suggested that miR-181c may serve as an important therapeutic target for NB patients.

References

Oct 24, 1998·The Journal of Biological Chemistry·S ItóhP ten Dijke
Feb 20, 2004·Proceedings of the National Academy of Sciences of the United States of America·George Adrian CalinCarlo M Croce
Sep 17, 2004·Nature·Victor Ambros
Dec 8, 2005·Journal of the National Cancer Institute·Haruhito AzumaShigeo Horie
Mar 3, 2007·Cancer Research·Delphine JavelaudAlain Mauviel
Sep 11, 2008·British Journal of Cancer·S K HalderP K Datta
Oct 29, 2008·Genome Research·Robin C FriedmanDavid P Bartel
Jul 9, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Junfang JiXin Wei Wang
Feb 12, 2010·Cancer Biology & Therapy·Jigang GuoChen-Yu Zhang
Aug 2, 2011·Seminars in Nuclear Medicine·Susan E SharpBarry L Shulkin
Jan 29, 2013·International Journal of Cardiology·Jian LiGuo-ying Huang
Mar 8, 2013·PloS One·Jingbo QiaoDai H Chung

❮ Previous
Next ❯

Citations

Oct 17, 2015·International Journal of Molecular Sciences·Cuiying LiuDongguo Li
Mar 16, 2019·RNA Biology·Lan-Fang SunZi-Bing Jin
Jun 11, 2015·Cellular and Molecular Life Sciences : CMLS·Pak-Yin LuiNigel J Stevenson
Jun 18, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Lingyu LuoDeqiang Huang
Jun 13, 2016·Cell and Tissue Research·Bert DhondtAn Hendrix
Apr 28, 2020·NPJ Genomic Medicine·Vito Alessandro LasorsaMario Capasso
Dec 19, 2019·Cancer Metastasis Reviews·Karina Bezerra SalomãoMaría Sol Brassesco
Jun 13, 2015·Oncotarget·Federica MoriSabrina Strano
Mar 22, 2020·International Journal of Molecular Sciences·Alessia IndrieriBrunella Franco
Oct 22, 2020·Critical Reviews in Biochemistry and Molecular Biology·Charlotte de Ceuninck van CapellePeter Ten Dijke
Feb 14, 2021·NPJ Genomic Medicine·Vito Alessandro LasorsaMario Capasso
Aug 27, 2021·Oncotarget·Nivaldo Faria VieiraJosé Sebastião Dos Santos

❮ Previous
Next ❯

Related Concepts