miR-181d-5p-FOXP1 feedback loop modulates the progression of osteosarcoma

Biochemical and Biophysical Research Communications
Haojie ChenXiaodong Zhu

Abstract

MicroRNAs (miRNAs) are playing more and more fundamental roles in the progress of human cancers. miR-181d-5p has not been fully elucidated in human cancers. In this study, we aimed to investigate the biological function and mechanism of miR-181d-5p in osteosarcoma (OS). At first, the expression conditions of miR-181d-5p were identified in OS tissues and cell lines. Downregulation of miR-181d-5p was identified to be a significant prognostic factor for patients with OS. Using the bioinformatics analysis, the putative target mRNA (FOXP1) of miR-181d-5p was found out. Similarly, the expression conditions and prognostic value of FOXP1 were identified in OS. To validate the tumor suppressive role of miR-181d-5p in OS, gain or loss of function assays were carried out in OS cell lines. The results indicated the anti-oncogenic function of miR-181d-5p in OS. Subsequently, the oncogenic function of FOXP1 in OS was identified by functional assays. Rescue assays manifested that the oncogenic function of FOXP1 can be partially reversed by miR-181d-5p. Combining with the result of luciferase reporter assay, we confirmed that miR-181d-5p can act as a tumor suppressor in OS via targeting FOXP1. Further mechanism experiments revealed that FOXP1 ...Continue Reading

Citations

Apr 6, 2019·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·G M VieraM S Brassesco
Jun 14, 2019·Journal of Cellular Physiology·Hongbo SunLin Wu
Oct 28, 2020·Toxicological Sciences : an Official Journal of the Society of Toxicology·Qiuhan HuaYiguo Jiang
Feb 14, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Yanping LiuYe Lin

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