MiR-187 suppresses non-small-cell lung cancer cell proliferation by targeting FGF9.

Bioengineered
Zhihua LiangWanhong Zhu

Abstract

Non-small-cell lung cancer (NSCLC) is the main pathological type of lung cancer and has a low overall five-year survival rate. miR-187 has been reported to play major roles in various tumor types. In this study, we explored the impact of miR-187 on NSCLC. qRT-PCR results demonstrated that miR-187 expression is lower in NSCLC and cancer cells than normal tissues and normal lung cells. miR-187 expression levels are associated with tumor size, TNM stage and overall survival rate. MTS and colony formation assays showed that high miR-187 expression inhibits NSCLC cell proliferation and colony formation ability, and flow cytometry showed that miR-187 overexpression induces cell cycle arrest at the G0/G1 phase. A luciferase reporter assay showed that FGF9 is a target of miR-187. miR-187 overexpression reduces the expression of FGF9, cyclin D1 CDK4 and CDK6. Therefore, miR-187 may present a new NSCLC treatment target by regulates cyclins-related protein expression.

References

Jul 19, 2005·Cancer Research·Jennifer A ChanKenneth S Kosik
Feb 14, 2008·The Journal of Clinical Endocrinology and Metabolism·Marina N NikiforovaYuri E Nikiforov
Oct 14, 2008·Journal of Occupational and Environmental Medicine·Sania AmrCurtis C Harris
Nov 19, 2013·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Keiko OhginoTomoko Betsuyaku
Feb 5, 2016·Biochemical and Biophysical Research Communications·Chengcao SunDejia Li
Mar 10, 2016·Journal of the National Comprehensive Cancer Network : JNCCN·David S EttingerMiranda Hughes
Jun 3, 2016·CA: a Cancer Journal for Clinicians·Kimberly D MillerAhmedin Jemal
Aug 9, 2016·Biochemical and Biophysical Research Communications·Ming MaoJiakuan Chen
Jul 22, 2017·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Byoung Chul ChoAnna Wrona
Aug 16, 2017·Experimental and Therapeutic Medicine·Chengliang Cui, Xiaoyu Shi
Aug 30, 2017·Oncology Reports·Hua LiangAili Tan
Jan 6, 2018·Cancer Investigation·Yi XiaoDao-Zheng Zhou
Jan 10, 2018·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
Sep 6, 2018·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Ye YuanAnke van den Berg
Sep 16, 2018·Journal of Experimental & Clinical Cancer Research : CR·Hongmei ZhengSongqing Fan
Oct 26, 2018·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Min LiHong-Bo Wang
Oct 28, 2018·Journal of Cellular Physiology·Taicheng ZhouXiaoyun Mao
Nov 18, 2018·Biochemical and Biophysical Research Communications·Rongjiong ZhengMeiling Hu
Nov 20, 2018·Cancer Biomarkers : Section a of Disease Markers·Qianfeng ZhuangJianquan Hou
Nov 21, 2018·Current Topics in Medicinal Chemistry·Sumit Biswas
Nov 21, 2018·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Zhonghu LiXiangde Liu
Feb 21, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yudi XiongYunfeng Zhou
Jun 19, 2019·Bioengineered·Bing Liu, Xuerong Sun

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Citations

Jun 9, 2020·Cancer Biomarkers : Section a of Disease Markers·Juan ChenJun Qi

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Methods Mentioned

BETA
xenograft
PCR
transfection
electrophoresis
xenografts
flow cytometry
FACS

Software Mentioned

SPSS

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