miR-192 suppresses T follicular helper cell differentiation by targeting CXCR5 in childhood asthma

Scandinavian Journal of Clinical and Laboratory Investigation
Defeng ZhangGengyun Sun

Abstract

The aim of this study was to investigate the role of miR-192 in differentiation of T follicular helper cells in childhood asthma. Blood samples were taken from eighteen children with acute asthma attacks and fifteen healthy children (HC). Quantitative real-time PCR and Western blotting were used to detect the expression levels of miR-192, C-X-C chemokine receptor type 5 (CXCR5), B-cell lymphoma 6 (BCL-6) and inducible T-cell costimulator (ICOS). The flow cytometry was performed to detect the proportion of CD4 + CXCR5+ Tfh cells on CD4 + T lymphocytes. The enzyme-linked immunosorbent assay (ELISA) was carried out to determine the plasma concentrations of total IgE and IL-21. The effect of miR-192 on the T follicular helper cells differentiation by targeting CXCR5 was determined by dual-luciferase reporter assay. Children with asthma had lower levels of miR-192 than HC. The proportion of CD4 + CXCR + Tfh cells was significantly higher in the acute asthma group than HC. Similarly, the plasma concentration of total IgE and IL-21 in the acute group markedly increased compared with the HC, and IgE concentration was positively correlated with the proportion of CD4 + CXCR5 + Tfh cells. Furthermore, the expression levels of CXCR5, Bcl-6...Continue Reading

References

Jan 30, 2003·RNA·Mariana Lagos-QuintanaThomas Tuschl
Jul 13, 2004·Allergy·E J O'Connell
Jan 5, 2008·Annual Review of Immunology·Cecile KingCharles R Mackay
Nov 19, 2011·Nature Reviews. Genetics·Manel Esteller
Feb 10, 2012·The Journal of Asthma : Official Journal of the Association for the Care of Asthma·Sarah H Y KamScott J Tebbutt
Feb 22, 2012·European Journal of Epidemiology·Liesbeth Duijts
May 3, 2012·Nature Reviews. Rheumatology·Joseph E Craft
May 18, 2013·Nature Reviews. Immunology·Stuart G TangyeElissa K Deenick
May 23, 2013·Seminars in Immunology·Inken SchmuddeJörg Köhl
May 30, 2013·Current Vascular Pharmacology·Pasquale FasanaroMaurizio C Capogrossi
Sep 5, 2013·Allergy, Asthma & Immunology Research·Gary W K WongFanny W S Ko
Oct 22, 2013·Immunity·Nathalie Schmitt, Hideki Ueno
Nov 14, 2013·PloS One·Galina Feinberg-GorenshteinSmadar Avigad
Mar 29, 2014·Annals of the American Thoracic Society·Thomas Ferkol, Dean Schraufnagel
Jul 8, 2014·Trends in Immunology·Nathalie SchmittHideki Ueno
Aug 27, 2014·Current Opinion in Immunology·Lawren C Wu, Heleen Scheerens
Oct 10, 2014·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·Anne Dorthea RøTorbjørn Øien
Dec 6, 2014·Arthritis Research & Therapy·Irene Arroyo-VillaMaría-Eugenia Miranda-Carús
Apr 12, 2016·Breathe
Dec 10, 2016·Allergy and Asthma Proceedings·Fang GongDongLin Jiang
May 11, 2017·American Journal of Respiratory Cell and Molecular Biology·Enrico HefflerSebastiano Gangemi
Jun 29, 2017·Immunological Reviews·Damon J TumesToshinori Nakayama
Aug 16, 2017·Experimental and Therapeutic Medicine·Xiaojuan MaJianbing Ding
Aug 18, 2017·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·Manuel Ferreira de MagalhãesJoão A Fonseca

❮ Previous
Next ❯

Citations

May 18, 2019·Journal of Cellular Physiology·Seyed Reza MousaviJafar Salimian
Nov 20, 2019·Allergy, Asthma & Immunology Research·Styliani TakaNikolaos G Papadopoulos
Oct 18, 2020·International Journal of Molecular Sciences·Andrés TittarelliFlavio Salazar-Onfray
Nov 26, 2020·Current Allergy and Asthma Reports·Tiancong LiuWeiliang Bai
Dec 29, 2020·Therapeutic Advances in Respiratory Disease·Li XuYuan Zhang
Mar 13, 2021·Frontiers in Pharmacology·Fu-Jia RenGuo-Ying Fang
Aug 11, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Soudeh Ghafouri-FardMarek Sanak

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allergies & Environmental Factors

Environmental factors are strongly associated with the prevalence of allergies and are an increasing health concern worldwide. Discover the latest research on Allergies and Environmental Factors here.

Asthma

This feed focuses in Asthma in which your airways narrow and swell. This can make breathing difficult and trigger coughing, wheezing and shortness of breath.

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.

B-Cell Lymphoma

B-cell lymphomas include lymphomas that affect B cells. This subtype of cancer accounts for over 80% of non-Hodgkin lymphomas in the US. Here is the latest research.