MiR-193a-5p and -3p Play a Distinct Role in Gastric Cancer: miR-193a-3p Suppresses Gastric Cancer Cell Growth by Targeting ETS1 and CCND1

Anticancer Research
Nan-Hua ChouKuo-Wang Tsai

Abstract

MicroRNAs (miRNAs) are small non-protein-coding RNAs, that can be generated from the 5p or 3p arm of precursor miRNA (pre-miRNA). Differential miRNA arm selection has been reported between tumor and normal tissue in many cancer types; however, the biological function and mechanism of miRNA arm switching in gastric cancer remain unclear. Profiles of miRNA expression in gastric cancer were obtained from The Cancer Genome Atlas (TCGA). The biological role of miR-193a-5p/-3p in tumor growth and invasive abilities was assessed through a gain-of-function approach. Target genes of miR-193a-3p were identified using bioinformatics and an experimental approach. The expression levels of miR-193a-5p, and not of miR-193a-3p, were significantly decreased in gastric cancer compared to adjacent normal tissues. Ectopic expressions of miR-193a-5p and miR-193a-3p revealed that they both inhibited gastric cancer cell growth, but only miR-193a-3p significantly suppressed cell invasion ability. Using a bioinformatics approach, we identified 18 putative target genes of miR-193a-3p. Both mRNA and protein levels of cyclin D1 (CCND1) and ETS proto-oncogene 1 (ETS1) were significantly decreased in AGS cells transfected with miR-193a-3p mimics. ETS1 or CC...Continue Reading

Citations

Apr 25, 2019·International Journal of Molecular Medicine·Yiyin TangQian Zhang
May 7, 2020·International Journal of Molecular Sciences·Olga A EfimovaAnna A Pendina
Aug 14, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Bing-Ze LinYi-Jang Lee
Sep 2, 2020·International Journal of Molecular Sciences·Beatrice PoliniPaola Nieri
Oct 31, 2020·Frontiers in Endocrinology·Gabriella Simões HeynKelly Grace Magalhães

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