MiR-194-5p inhibited metastasis and EMT of nephroblastoma cells through targeting Crk

The Kaohsiung Journal of Medical Sciences
Hong LiuHong Ma

Abstract

Wilms tumor (WT) is the most common solid childhood tumors all over the world. MicroRNAs (miRs) contribute to tumorigenesis of various cancers through targeting gene. The present study investigated the vital role of miR-194-5p and its underlying mechanism in the progression of WT. Immunohistochemistry and quantitative real-time polymerase chain reaction (qRT-PCR) assay indicated downregulation of miR194-5p and upregulation of Crk, in WT tissues compared to adjacent normal tissues. Transfection with miR-194-5p mimics into nephroblastoma cells showed a significant decline in cell migration and invasion, which was detected by Transwell assay. Luciferase assay confirmed that Crk was a direct target gene of miR-194-5p. More important, the mesenchymal to epithelial transition (EMT) biomarkers containing E-cadherin, N-cadherin and Zeb1 were examined by Western blot, and revealed that miR-194-5p mimics decreased the levels of N-cadherin and Zeb1 but increased E-cadherin, which suggested that miR-194-5p inhibited EMT. Crk knockdown could reverse the increased nephroblastoma cell invasion, migration and EMT caused by miR-194-5p inhibitor. Interestingly, qRT-PCR and Western blot analysis showed that overexpression of miR-194-5p deactivate...Continue Reading

References

Aug 1, 1992·Molecular and Cellular Biology·M MatsudaM Shibuya
Jan 1, 1991·Pediatrics in Review·A E Brodeur, G M Brodeur
Jan 1, 1993·Medical and Pediatric Oncology·N BreslowD M Green
Apr 16, 2005·Molecular Cancer Research : MCR·Sonia P RodriguesMorag Park
Aug 20, 2005·Nature Reviews. Cancer·Miguel N Rivera, Daniel A Haber
Sep 11, 2007·Biochemical and Biophysical Research Communications·Lei WangShinya Tanaka
May 12, 2009·Cell Communication and Signaling : CCS·Raymond B BirgeShinya Tanaka
Jul 14, 2010·Cancer Letters·Runhua FengZhenggang Zhu
Oct 28, 2010·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Zhipeng MengWendong Huang
Dec 20, 2011·Nature Cell Biology·Kathryn AustgenScott A Oakes
May 10, 2012·Breast Cancer Research : BCR·Kelly E FathersMorag Park
Jul 14, 2012·Advances in Pediatrics·Andrew M Davidoff
May 31, 2014·Nature Cell Biology·Alain PuisieuxJulie Caramel
Aug 7, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Xudong ChenGuohua Song
Nov 19, 2015·Acta Pharmaceutica Sinica. B·Lihua Huang, Liwu Fu
Dec 15, 2015·Nature Chemical Biology·Tamjeed SalehCharalampos G Kalodimos
Jun 3, 2016·Journal of Human Genetics·Keiichi YonemoriShoji Natsugoe
May 13, 2017·Molecular Medicine Reports·Liyi HongHong Zhu
May 23, 2017·The Tohoku Journal of Experimental Medicine·Mingyu CuiRongde Wu
Sep 5, 2018·Biochemical and Biophysical Research Communications·Yongzhen LiNa Li

❮ Previous
Next ❯

Citations

Apr 23, 2021·Cancer Management and Research·Xingyue HeGuanghui Wei
Jul 13, 2021·Archives of Biochemistry and Biophysics·Jayashree JayachandranKrishna Priya Mani
Aug 14, 2021·Technology in Cancer Research & Treatment·Wang LiLi Haisha
Sep 30, 2021·Expert Reviews in Molecular Medicine·Sepideh MirzaeiHaroon Khan

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.